Academic Journal
Characterization of Bioactive Actinomycetes Isolated from Kadolkele Mangrove Sediments, Sri Lanka
| العنوان: | Characterization of Bioactive Actinomycetes Isolated from Kadolkele Mangrove Sediments, Sri Lanka |
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| المؤلفون: | Naligama Kishani N., Weerasinghe Kavindi E., Halmillawewa Anupama P. |
| المصدر: | Polish Journal of Microbiology, Vol 71, Iss 2, Pp 191-204 (2022) |
| بيانات النشر: | Sciendo, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Genetics LCC:Microbiology |
| مصطلحات موضوعية: | mangrove sediment bacteria, bioactive actinomycetes, extracellular enzymes, bacterial pigments, Genetics, QH426-470, Microbiology, QR1-502 |
| الوصف: | Exploring untapped microbial potentials in previously uncharted environments has become crucial in discovering novel secondary metabolites and enzymes for biotechnological applications. Among prokaryotes, actinomycetes are well recognized for producing a vast range of secondary metabolites and extracellular enzymes. In the present study, we have used surface sediments from ‘Kadolkele’ mangrove ecosystem located in the Negombo lagoon area, Sri Lanka, to isolate actinomycetes with bioactive potentials. A total of six actinomycetes were isolated on modified-starch casein agar and characterized. The isolates were evaluated for their antibacterial activity against four selected bacterial strains and to produce extracellular enzymes: cellulase, amylase, protease, and lipase. Three out of the six isolates exhibited antibacterial activity against Staphylococcus aureus, Escherichia coli, and Bacillus cereus, but not against Listeria monocytogenes. Five strains could produce extracellular cellulase, while all six isolates exhibited amylase activity. Only three of the six isolates were positive for protease and lipase assays separately. Ac-1, Ac-2, and Ac-9, identified as Streptomyces spp. with the 16S rRNA gene sequencing, were used for pigment extraction using four different solvents. Acetone-extracted crude pigments of Ac-1 |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2544-4646 |
| Relation: | https://doaj.org/toc/2544-4646 |
| DOI: | 10.33073/pjm-2022-017 |
| URL الوصول: | https://doaj.org/article/635efd897e994c4a92ee3eb373c1c2ae |
| رقم الانضمام: | edsdoj.635efd897e994c4a92ee3eb373c1c2ae |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 25444646 |
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| DOI: | 10.33073/pjm-2022-017 |