التفاصيل البيبلوغرافية
| العنوان: |
Antioxidant and anticancer effects in human hepatocarcinoma (HepG2) cells of papain-hydrolyzed sorghum kafirin hydrolysates |
| المؤلفون: |
Shiwei Xu, Yanting Shen, Jingwen Xu, Guangyan Qi, Gengjun Chen, Weiqun Wang, Xiuzhi Sun, Yonghui Li |
| المصدر: |
Journal of Functional Foods, Vol 58, Iss , Pp 374-382 (2019) |
| بيانات النشر: |
Elsevier, 2019. |
| سنة النشر: |
2019 |
| المجموعة: |
LCC:Nutrition. Foods and food supply |
| مصطلحات موضوعية: |
Sorghum kafirin, Protein hydrolysates, Peptide antioxidants, Anticancer effect, Emulsion, Meat systems, Nutrition. Foods and food supply, TX341-641 |
| الوصف: |
Peptide antioxidants are becoming increasingly popular in the food industry. This study reported antioxidant and anticancer properties of papain-hydrolyzed sorghum kafirin. The optimum hydrolysis conditions were determined to be 360 kU/g enzyme-to-substrate ratio, and 4 h of reaction time. After ultrafiltration, the 1–3 kDa hydrolysate fraction demonstrating promising antioxidative activities as well as yield was selected for further analyses. In an oil-in-water emulsion system, the incorporation of the selected fraction of hydrolysates at 10 mg/mL resulted in an average inhibition of primary and secondary oxidation products by 53.7% and 43.0%, respectively, without significantly changing emulsion turbidity or stability. In a ground meat system, an average of 32.1% inhibition towards lipid peroxidation was also detected when incorporated at 1.0 mg/g. The kafirin hydrolysates also effectively reduced HepG2 cell growth via non-toxic mechanisms, indicating its anticancer potential. Further, the 1–3 kDa peptides were fractionated and 13 peptide sequences were identified using RP-HPLC analysis followed by MALDI-TOF/TOF MS from the most potent fraction. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1756-4646 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S1756464619302725; https://doaj.org/toc/1756-4646 |
| DOI: |
10.1016/j.jff.2019.05.016 |
| URL الوصول: |
https://doaj.org/article/634c37303b4d4ad0b463286f9a16b2bf |
| رقم الانضمام: |
edsdoj.634c37303b4d4ad0b463286f9a16b2bf |
| قاعدة البيانات: |
Directory of Open Access Journals |