Academic Journal
Determination and Pharmacokinetics of Di-(2-ethylhexyl) Phthalate in Rats by Ultra Performance Liquid Chromatography with Tandem Mass Spectrometry
| العنوان: | Determination and Pharmacokinetics of Di-(2-ethylhexyl) Phthalate in Rats by Ultra Performance Liquid Chromatography with Tandem Mass Spectrometry |
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| المؤلفون: | Tung-Hu Tsai, Lie-Chwen Lin, Li-Wen Chang, Yin-Meng Tsai, Chia-Jung Lee, Mei-Ling Hou, Wan-Ling Chang-Liao |
| المصدر: | Molecules, Vol 18, Iss 9, Pp 11452-11466 (2013) |
| بيانات النشر: | MDPI AG, 2013. |
| سنة النشر: | 2013 |
| المجموعة: | LCC:Organic chemistry |
| مصطلحات موضوعية: | di-(2-ethylhexyl) phthalate (DEHP), fecal excretion, pharmacokinetics, phthalates, plasticizer, Organic chemistry, QD241-441 |
| الوصف: | Di-(2-ethylhexyl) phthalate (DEHP) is used to increase the flexibility of plastics for industrial products. However, the illegal use of the plasticizer DEHP in food and drinks has been reported in Taiwan in 2011. In order to assess the exact extent of the absorption of DEHP via the oral route, the aim of this study is to develop a reliable and validated ultra performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) method to evaluate the oral bioavailability of DEHP in rats. The optimal chromatographic separation of DEHP and butyl benzyl phthalate (BBP; used as internal standard) were achieved on a C18 column. The mobile phase was consisted of 5 mM ammonium acetate-methanol (11:89, v/v) with a flow rate of 0.25 mL/min. The monitoring ion transitions were m/z 391.4 → 149.0 for DEHP and m/z 313.3 → 149.0 for BBP. The mean matrix effects of DEHP at low, medium and high concentrations were 94.5 ± 5.7% and 100.1 ± 2.3% in plasma and feces homogenate samples, respectively. In conclusion, the validated UPLC-MS/MS method is suitable for analyzing the rat plasma sample of DEHP and the oral bioavailability of DEHP was about 7% in rats. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1420-3049 91224497 |
| Relation: | http://www.mdpi.com/1420-3049/18/9/11452; https://doaj.org/toc/1420-3049 |
| DOI: | 10.3390/molecules180911452 |
| URL الوصول: | https://doaj.org/article/625a440d9122449798dbc648b6856524 |
| رقم الانضمام: | edsdoj.625a440d9122449798dbc648b6856524 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14203049 91224497 |
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| DOI: | 10.3390/molecules180911452 |