التفاصيل البيبلوغرافية
| العنوان: |
A bHLH transcription factor, CsSPT, regulates high-temperature resistance in cucumber |
| المؤلفون: |
Yonggui Liang, Chenyu Yang, Fangyan Ming, Bingwei Yu, Zhihua Cheng, Yixi Wang, Zhengkun Qiu, Xiaolan Zhang, Bihao Cao, Shuangshuang Yan |
| المصدر: |
Horticultural Plant Journal, Vol 10, Iss 2, Pp 503-514 (2024) |
| بيانات النشر: |
KeAi Communications Co., Ltd., 2024. |
| سنة النشر: |
2024 |
| المجموعة: |
LCC:Plant culture |
| مصطلحات موضوعية: |
Cucumber, bHLH, CsSPT, Photosynthesis, High temperature, Plant culture, SB1-1110 |
| الوصف: |
High-temperature stress threatens the growth and yield of crops. Basic helix-loop-helix (bHLH) transcription factors (TFs) have been shown to play important roles in regulating high-temperature resistance in plants. However, the bHLH TFs responsible for high-temperature tolerance in cucumbers have not been identified. We used transcriptome profiling to screen the high temperature-responsive candidate bHLH TFs in cucumber. Here, we found that the expression of 75 CsbHLH genes was altered under high-temperature stress. The expression of the CsSPT gene was induced by high temperatures in TT (Thermotolerant) cucumber plants. However, the Csspt mutant plants obtained by the CRISPR-Cas9 system showed severe thermosensitive symptoms, including wilted leaves with brown margins and reduced root density and cell activity. The Csspt mutant plants also exhibited elevated H2O2 levels and down-regulated photosystem-related genes under normal conditions. Furthermore, there were high relative electrolytic leakage (REC), malondialdehyde (MDA), glutathione (GSH), and superoxide radical (O2·−) levels in the Csspt mutant plants, with decreased Proline content after the high-temperature treatment. Transcriptome analysis showed that the photosystem and chloroplast activities in Csspt mutant plants were extremely disrupted by the high-temperature stress compared with wild-type (WT) plants. Moreover, the plant hormone signal transduction, as well as MAPK and calcium signaling pathways were activated in Csspt mutant plants under high-temperature stress. The HSF and HSP family genes shared the same upregulated expression patterns in Csspt and WT plants under high-temperature conditions. However, most bHLH, NAC, and bZIP family genes were significantly down-regulated by heat in Csspt mutant plants. Thus, these results demonstrated that CsSPT regulated the high-temperature response by recruiting photosynthesis components, signaling pathway molecules, and transcription factors. Our results provide important insights into the heat response mechanism of CsSPT in cucumber and its potential as a target for breeding heat-resistant crops. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2468-0141 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2468014123000250; https://doaj.org/toc/2468-0141 |
| DOI: |
10.1016/j.hpj.2023.02.005 |
| URL الوصول: |
https://doaj.org/article/61f4c14e736949ac93c9ae1540ddbdac |
| رقم الانضمام: |
edsdoj.61f4c14e736949ac93c9ae1540ddbdac |
| قاعدة البيانات: |
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