التفاصيل البيبلوغرافية
| العنوان: |
In Vivo and In Vitro Pro-Fibrotic Response of Lung-Resident Mesenchymal Stem Cells from Patients with Idiopathic Pulmonary Fibrosis |
| المؤلفون: |
Gabriel Escarrer-Garau, Aina Martín-Medina, Joan Truyols-Vives, Cristina Gómez-Bellvert, Linda Elowsson, Gunilla Westergren-Thorsson, Maria Molina-Molina, Josep Mercader-Barceló, Ernest Sala-Llinàs |
| المصدر: |
Cells, Vol 13, Iss 2, p 160 (2024) |
| بيانات النشر: |
MDPI AG, 2024. |
| سنة النشر: |
2024 |
| المجموعة: |
LCC:Cytology |
| مصطلحات موضوعية: |
idiopathic pulmonary fibrosis, lung-resident mesenchymal stem cell, transforming growth factor β, bleomycin, inflammation, extracellular matrix proteins, Cytology, QH573-671 |
| الوصف: |
Lung-resident mesenchymal stem cells (LR-MSC) are thought to participate in idiopathic pulmonary fibrosis (IPF) by differentiating into myofibroblasts. On the other hand, LR-MSC in IPF patients present senescence-related features. It is unclear how they respond to a profibrotic environment. Here, we investigated the profibrotic response of LR-MSC isolated from IPF and control (CON) patients. LR-MSC were inoculated in mice 48 h after bleomycin (BLM) instillation to analyze their contribution to lung damage. In vitro, LR-MSC were exposed to TGFβ. Mice inoculated with IPF LR-MSC exhibited worse maintenance of their body weight. The instillation of either IPF or CON LR-MSC sustained BLM-induced histological lung damage, bronchoalveolar lavage fluid cell count, and the expression of the myofibroblast marker, extracellular matrix (ECM) proteins, and proinflammatory cytokines in the lungs. In vitro, IPF LR-MSC displayed higher basal protein levels of aSMA and fibronectin than CON LR-MSC. However, the TGFβ response in the expression of TGFβ, aSMA, and ECM genes was attenuated in IPF LR-MSC. In conclusion, IPF LR-MSC have acquired myofibroblastic features, but their capacity to further respond to profibrotic stimuli seems to be attenuated. In an advanced stage of the disease, LR-MSC may participate in disease progression owing to their limited ability to repair epithelial damage. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2073-4409 |
| Relation: |
https://www.mdpi.com/2073-4409/13/2/160; https://doaj.org/toc/2073-4409 |
| DOI: |
10.3390/cells13020160 |
| URL الوصول: |
https://doaj.org/article/6138692926f04a3bb494e28fe6e54efa |
| رقم الانضمام: |
edsdoj.6138692926f04a3bb494e28fe6e54efa |
| قاعدة البيانات: |
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