Academic Journal
Presence of SARS-CoV-2 in urban effluents in southeast Buenos Aires, Argentina, May 2020 to March 2022
العنوان: | Presence of SARS-CoV-2 in urban effluents in southeast Buenos Aires, Argentina, May 2020 to March 2022 |
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المؤلفون: | Carlos Cimmino, Leandro Rodrigues Capítulo, Andrea Lerman, Andrea Silva, Gabriela Von Haften, Ana P. Comino, Luciana Cigoy, Marcelo Scagliola, Verónica Poncet, Gonzalo Caló, Osvaldo Uez, Corina M. Berón |
المصدر: | Revista Panamericana de Salud Pública, Vol 47, Iss 94, Pp 1-11 (2023) |
بيانات النشر: | Pan American Health Organization, 2023. |
سنة النشر: | 2023 |
المجموعة: | LCC:Medicine LCC:Arctic medicine. Tropical medicine LCC:Public aspects of medicine |
مصطلحات موضوعية: | sars-cov-2, wastewater, disease outbreaks, environmental monitoring, argentina, Medicine, Arctic medicine. Tropical medicine, RC955-962, Public aspects of medicine, RA1-1270 |
الوصف: | Objectives. To implement and evaluate the use of wastewater sampling for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in two coastal districts of Buenos Aires Province, Argentina. Methods. In General Pueyrredon district, 400 mL of wastewater samples were taken with an automatic sampler for 24 hours, while in Pinamar district, 20 L in total (2.2 L at 20-minute intervals) were taken. Samples were collected once a week. The samples were concentrated based on flocculation using polyaluminum chloride. RNA purification and target gene amplification and detection were performed using reverse transcription polymerase chain reaction for clinical diagnosis of human nasopharyngeal swabs. Results. In both districts, the presence of SARS-CoV-2 was detected in wastewater. In General Pueyrredon, SARS-CoV-2 was detected in epidemiological week 28, 2020, which was 20 days before the start of an increase in coronavirus virus disease 2019 (COVID-19) cases in the first wave (epidemiological week 31) and 9 weeks before the maximum number of laboratory-confirmed COVID-19 cases was recorded. In Pinamar district, the virus genome was detected in epidemiological week 51, 2020 but it was not possible to carry out the sampling again until epidemiological week 4, 2022, when viral circulation was again detected. Conclusions. It was possible to detect SARS-CoV-2 virus genome in wastewater, demonstrating the usefulness of the application of wastewater epidemiology for long-term SARS-CoV-2 detection and monitoring. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English Spanish; Castilian Portuguese |
تدمد: | 1020-4989 1680-5348 |
Relation: | https://iris.paho.org/handle/10665.2/57667; https://doaj.org/toc/1020-4989; https://doaj.org/toc/1680-5348 |
DOI: | 10.26633/RPSP.2023.94 |
URL الوصول: | https://doaj.org/article/607599d408224ff0b416308b84ecba84 |
رقم الانضمام: | edsdoj.607599d408224ff0b416308b84ecba84 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 10204989 16805348 |
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DOI: | 10.26633/RPSP.2023.94 |