Academic Journal
Uncoupling protein-2 mRNA expression in mice subjected to intermittent hypoxia
| العنوان: | Uncoupling protein-2 mRNA expression in mice subjected to intermittent hypoxia |
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| المؤلفون: | Luciana Rodrigues Vieira, Denis Martinez, Luiz Felipe Forgiarini, Darlan Pase da Rosa, Gustavo Alfredo Ochs de Muñoz, Micheli Fagundes, Emerson Ferreira Martins, Carolina Caruccio Montanari, Cintia Zappe Fiori |
| المصدر: | Jornal Brasileiro de Pneumologia, Vol 41, Iss 2, Pp 167-174 (2015) |
| بيانات النشر: | Sociedade Brasileira de Pneumologia e Tisiologia, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Diseases of the respiratory system |
| مصطلحات موضوعية: | Glicemia, Síndromes da apneia do sono, Pâncreas, Células secretoras de glucagon, Diseases of the respiratory system, RC705-779 |
| الوصف: | Objective: To investigate the effect of intermittent hypoxia-a model of obstructive sleep apnea (OSA)-on pancreatic expression of uncoupling protein-2 (UCP2), as well as on glycemic and lipid profiles, in C57BL mice. Methods: For 8 h/day over a 35-day period, male C57BL mice were exposed to intermittent hypoxia (hypoxia group) or to a sham procedure (normoxia group). The intermittent hypoxia condition involved exposing mice to an atmosphere of 92% N and 8% CO2 for 30 s, progressively reducing the fraction of inspired oxygen to 8 ± 1%, after which they were exposed to room air for 30 s and the cycle was repeated (480 cycles over the 8-h experimental period). Pancreases were dissected to isolate the islets. Real-time PCR was performed with TaqMan assays. Results: Expression of UCP2 mRNA in pancreatic islets was 20% higher in the normoxia group than in the hypoxia group (p = 0.11). Fasting serum insulin was higher in the hypoxia group than in the normoxia group (p = 0.01). The homeostasis model assessment of insulin resistance indicated that, in comparison with the control mice, the mice exposed to intermittent hypoxia showed 15% lower insulin resistance (p = 0.09) and 21% higher pancreatic β-cell function (p = 0.01). Immunohistochemical staining of the islets showed no significant differences between the two groups in terms of the area or intensity of α- and β-cell staining for insulin and glucagon. Conclusions: To our knowledge, this is the first report of the effect of intermittent hypoxia on UCP2 expression. Our findings suggest that UCP2 regulates insulin production in OSA. Further study of the role that UCP2 plays in the glycemic control of OSA patients is warranted. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Portuguese |
| تدمد: | 1806-3756 1806-3713 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-37132015000200167&lng=en&tlng=en; https://doaj.org/toc/1806-3756 |
| DOI: | 10.1590/S1806-37132015000004414 |
| URL الوصول: | https://doaj.org/article/a602cd88833045e88c78f29cdf50336d |
| رقم الانضمام: | edsdoj.602cd88833045e88c78f29cdf50336d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 18063756 18063713 |
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| DOI: | 10.1590/S1806-37132015000004414 |