Academic Journal
Candida albicans Adhesins Als1 and Hwp1 Modulate Interactions with Streptococcus mutans
| العنوان: | Candida albicans Adhesins Als1 and Hwp1 Modulate Interactions with Streptococcus mutans |
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| المؤلفون: | Loyse Martorano-Fernandes, James S. Goodwine, Antônio Pedro Ricomini-Filho, Clarissa J. Nobile, Altair Antoninha Del Bel Cury |
| المصدر: | Microorganisms, Vol 11, Iss 6, p 1391 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | Candida albicans, Streptococcus mutans, biofilms, polymicrobial biofilms, dual-species biofilms, oral cavity, Biology (General), QH301-705.5 |
| الوصف: | Candida albicans and Streptococcus mutans are known to synergistically interact with each other in the oral cavity. For example, glucosyltransferase B (GtfB), secreted by S. mutans, can bind to the C. albicans cell surface, promoting dual-species biofilm formation. However, the fungal factors mediating interactions with S. mutans are unknown. The C. albicans adhesins Als1, Als3, and Hwp1 are key players in C. albicans single-species biofilm formation, but their roles, if any, in interacting with S. mutans have not been assessed. Here, we investigated the roles of the C. albicans cell wall adhesins Als1, Als3, and Hwp1 on forming dual-species biofilms with S. mutans. We assessed the abilities of the C. albicans wild-type als1Δ/Δ, als3Δ/Δ, als1Δ/Δ/als3Δ/Δ, and hwp1Δ/Δ strains to form dual-species biofilms with S. mutans by measuring optical density, metabolic activity, cell enumeration, biomass, thickness, and architecture of the biofilms. We observed that the C. albicans wild-type strain formed enhanced dual-species biofilms in the presence of S. mutans in these different biofilm assays, confirming that C. albicans and S. mutans synergistically interact in the context of biofilms. Our results reveal that C. albicans Als1 and Hwp1 are major players in interacting with S. mutans, since dual-species biofilm formation was not enhanced when the als1Δ/Δ or hwp1Δ/Δ strains were cultured with S. mutans in dual-species biofilms. Als3, however, does not seem to play a clear role in interacting with S. mutans in dual-species biofilm formation. Overall, our data suggest that the C. albicans adhesins Als1 and Hwp1 function to modulate interactions with S. mutans and could be potential targets for future therapeutics. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 11061391 2076-2607 |
| Relation: | https://www.mdpi.com/2076-2607/11/6/1391; https://doaj.org/toc/2076-2607 |
| DOI: | 10.3390/microorganisms11061391 |
| URL الوصول: | https://doaj.org/article/5f2a9fb9f9d14ceab29e242d63a271bc |
| رقم الانضمام: | edsdoj.5f2a9fb9f9d14ceab29e242d63a271bc |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 11061391 20762607 |
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| DOI: | 10.3390/microorganisms11061391 |