التفاصيل البيبلوغرافية
| العنوان: |
Microbial Cell Factory of Baccatin III Preparation in Escherichia coli by Increasing DBAT Thermostability and in vivo Acetyl-CoA Supply |
| المؤلفون: |
Jia-jun Huang, Tao Wei, Zhi-wei Ye, Qian-wang Zheng, Bing-hua Jiang, Wen-feng Han, An-qi Ye, Pei-yun Han, Li-qiong Guo, Jun-fang Lin |
| المصدر: |
Frontiers in Microbiology, Vol 12 (2022) |
| بيانات النشر: |
Frontiers Media S.A., 2022. |
| سنة النشر: |
2022 |
| المجموعة: |
LCC:Microbiology |
| مصطلحات موضوعية: |
baccatin III, 10-deacetylbaccatin III-10-O-transferase, gene integration, semi-rational design, thermostability, acetyl-CoA supplement, Microbiology, QR1-502 |
| الوصف: |
Given the rapid development of genome mining in this decade, the substrate channel of paclitaxel might be identified in the near future. A robust microbial cell factory with gene dbat, encoding a key rate-limiting enzyme 10-deacetylbaccatin III-10-O-transferase (DBAT) in paclitaxel biosynthesis to synthesize the precursor baccatin III, will lay out a promising foundation for paclitaxel de novo synthesis. Here, we integrated gene dbat into the wild-type Escherichia coli BW25113 to construct strain BWD01. Yet, it was relatively unstable in baccatin III synthesis. Mutant gene dbatS189V with improved thermostability was screened out from a semi-rational mutation library of DBAT. When it was over-expressed in an engineered strain N05 with improved acetyl-CoA generation, combined with carbon source optimization of fermentation engineering, the production level of baccatin III was significantly increased. Using this combination, integrated strain N05S01 with mutant dbatS189V achieved a 10.50-fold increase in baccatin III production compared with original strain BWD01. Our findings suggest that the combination of protein engineering and metabolic engineering will become a promising strategy for paclitaxel production. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1664-302X |
| Relation: |
https://www.frontiersin.org/articles/10.3389/fmicb.2021.803490/full; https://doaj.org/toc/1664-302X |
| DOI: |
10.3389/fmicb.2021.803490 |
| URL الوصول: |
https://doaj.org/article/e5f261d0bf88411fa73b66747999b50f |
| رقم الانضمام: |
edsdoj.5f261d0bf88411fa73b66747999b50f |
| قاعدة البيانات: |
Directory of Open Access Journals |
Full Text Finder