التفاصيل البيبلوغرافية
| العنوان: |
Comparison between the Roche Cobas 4800 Human Papillomavirus (HPV), Abbott RealTime High-Risk HPV, Seegene Anyplex II HPV28, and Novel Seegene Allplex HPV28 Assays for High-Risk HPV Detection and Genotyping in Mocked Self-Samples |
| المؤلفون: |
Margo Bell, Bo Verberckmoes, Janne Devolder, Heleen Vermandere, Olivier Degomme, Yasmin Medeiros Guimarães, Luani Rezende Godoy, Elena Ambrosino, Piet Cools, Elizaveta Padalko |
| المصدر: |
Microbiology Spectrum, Vol 11, Iss 4 (2023) |
| بيانات النشر: |
American Society for Microbiology, 2023. |
| سنة النشر: |
2023 |
| المجموعة: |
LCC:Microbiology |
| مصطلحات موضوعية: |
analytical performance, HPV genotyping, human papillomavirus, molecular diagnostics, Microbiology, QR1-502 |
| الوصف: |
ABSTRACT Infection with high-risk human papillomavirus (hrHPV) is well recognized as the main cause of cervical cancer. The recently developed Seegene Allplex HPV28 assay is a novel quantitative PCR (qPCR) assay designed to separately detect and quantify 28 distinct HPV genotypes in a fully automated and user-friendly manner. This study evaluated and compared the performance of this new assay with the performance of the Roche Cobas 4800, the Abbott RealTime high-risk HPV, and the Seegene Anyplex II HPV28 assays. A total of 114 mocked self-samples, i.e., semicervical samples collected by gynecologists using the Viba-Brush, were analyzed with all four HPV assays. Agreement in terms of detecting and genotyping HPV was assessed by the mean of the Cohen’s kappa (κ) coefficient. Results of all four HPV assays agreed in 85.9% of the cases when using the Abbott RealTime manufacturer’s recommended quantification cycle (Cq) cutoff for positivity ( |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2165-0497 |
| Relation: |
https://doaj.org/toc/2165-0497 |
| DOI: |
10.1128/spectrum.00081-23 |
| URL الوصول: |
https://doaj.org/article/5eb8f180eddc47c4a5129f42dda3c979 |
| رقم الانضمام: |
edsdoj.5eb8f180eddc47c4a5129f42dda3c979 |
| قاعدة البيانات: |
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