التفاصيل البيبلوغرافية
| العنوان: |
Isolation, culture, and use of primary murine myoblasts in small-molecule screens |
| المؤلفون: |
Yue Qu, Kaydine Edwards, Joeva Barrow |
| المصدر: |
STAR Protocols, Vol 4, Iss 2, Pp 102149- (2023) |
| بيانات النشر: |
Elsevier, 2023. |
| سنة النشر: |
2023 |
| المجموعة: |
LCC:Science (General) |
| مصطلحات موضوعية: |
Cell Biology, Cell Culture, Cell Isolation, Cell-based Assays, High-throughput Screening, Metabolism, Science (General), Q1-390 |
| الوصف: |
Summary: Small-molecule screens (SMS) are often performed using transformed cell lines that have limited physiological relevance to the biological system being investigated, resulting in poor translational outcomes. To circumvent this limitation, we present a protocol to perform SMS in primary murine myoblasts. We describe steps for isolating primary skeletal muscle myoblasts with greater than 95% purity, then describe techniques to establish a robust dynamic range, and conclude with steps to initiate a successful SMS.For complete details on the use and execution of this protocol, please refer to Richler and Yaffe (1970),1 Rando and Blau (1994),2 and Earle et al. (2020).3 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2666-1667 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2666166723001077; https://doaj.org/toc/2666-1667 |
| DOI: |
10.1016/j.xpro.2023.102149 |
| URL الوصول: |
https://doaj.org/article/e5eafa823eb54472bf68a06271368be7 |
| رقم الانضمام: |
edsdoj.5eafa823eb54472bf68a06271368be7 |
| قاعدة البيانات: |
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