التفاصيل البيبلوغرافية
| العنوان: |
Specificity and sensibility of primer pair in the detection of Colletotrichum gossypii var. cephalosporioides in cotton seeds by PCR technique |
| المؤلفون: |
Mirella Figueiró de Almeida, Sarah da Silva Costa, Iara Eleutéria Dias, Carolina da Silva Siqueira, José da Cruz Machado |
| المصدر: |
Journal of Seed Science, Vol 42 (2020) |
| بيانات النشر: |
Associação Brasileira de Tecnologia de Sementes, 2020. |
| سنة النشر: |
2020 |
| المجموعة: |
LCC:Plant culture |
| مصطلحات موضوعية: |
Colletotrichum gossypii, Colletotrichum gloeosporioides, Ramulosis, water restriction, Plant culture, SB1-1110 |
| الوصف: |
Abstract: Cotton Ramulosis (Gossypium hirsutum) is an important disease affecting cotton plantations in Brazil, and its causal agent, Colletotrichum gossypiivar.cephalosporioides(Cgc), according to the Brazilian phytosanitary authority, was considered a regulated non quarantine pest. It makes this microorganism subject to standardization in seed certification programs. The current seed health testing for detecting that pathogen in seed samples does not provide reliable results for routine analysis. On this paper, attempts were made to design specific primers for detection of Cgc associated with cotton seed. Two primer sets were selected based on the analysis of a multiple alignment of gene’s sequence encoding the glyceraldehyde 3-phosphate dehydrogenase from Cgc, C. gossypii and reference strains of the C. gloeosporioides species complex. The conserved sites unique to Cgc strains were used to design specific fragment of 140 bp. The primer specificity was confirmed by using other fungi. The primers produced a detectable band of target DNA of Cgc in all inoculum potentials of the pathogen artificially inoculated by the water restriction technique. The developed primer pair represents, therefore, a reliable and rapid mean to diagnose the Ramulosis agent in cotton seed. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2317-1545 |
| Relation: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372020000100202&tlng=en; https://doaj.org/toc/2317-1545 |
| DOI: |
10.1590/2317-1545v42229530 |
| URL الوصول: |
https://doaj.org/article/5da7382831c54fec83c0a9a1d2454594 |
| رقم الانضمام: |
edsdoj.5da7382831c54fec83c0a9a1d2454594 |
| قاعدة البيانات: |
Directory of Open Access Journals |