التفاصيل البيبلوغرافية
| العنوان: |
DedA Protein Relates to Action-Mechanism of Halicyclamine A, a Marine Spongean Macrocyclic Alkaloid, as an Anti-dormant Mycobacterial Substance |
| المؤلفون: |
Andi Setiawan, Motomasa Kobayashi, Takao Fujimoto, Liu Liu, Masayoshi Arai |
| المصدر: |
Marine Drugs, Vol 9, Iss 6, Pp 984-993 (2011) |
| بيانات النشر: |
MDPI AG, 2011. |
| سنة النشر: |
2011 |
| المجموعة: |
LCC:Biology (General) |
| مصطلحات موضوعية: |
halicyclamine A, dedA, marine sponge, dormant, tuberculosis, Biology (General), QH301-705.5 |
| الوصف: |
A macrocyclic alkaloid, halicyclamine A, was re-discovered from an Indonesian marine sponge of Haliclona sp. 05A08 as an anti-dormant mycobacterial substance. To clarify action-mechanism of halicyclamine A, halicyclamine A-resistant strains were screened from the transformants of Mycobacterium smegmatis with the genomic DNA library of M. bovis BCG, which were constructed in the multi-copy shuttle cosmid pYUB145. Sequencing analysis of the cosmids isolated from the halicyclamine A-resistant transformants revealed that the responsible gene was involved in the genome region between 2920.549 kb and 2933.210 kb. Further experiments using the transformants over-expressing individual gene contained in the responsible region were executed, and the transformant, which over-expressed BCG2664 gene assigned as dedA gene, was found to become halicyclamine A-resistant. This evidence strongly suggested that DedA protein correlates with the action-mechanism of halicyclamine A as an anti-dormant mycobacterial substance. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1660-3397 |
| Relation: |
http://www.mdpi.com/1660-3397/9/6/984/; https://doaj.org/toc/1660-3397 |
| DOI: |
10.3390/md9060984 |
| URL الوصول: |
https://doaj.org/article/5bc8fcb8825948db821ab321a98f40cf |
| رقم الانضمام: |
edsdoj.5bc8fcb8825948db821ab321a98f40cf |
| قاعدة البيانات: |
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