Academic Journal
Zingiber officinale-Derived Extracellular Vesicles Attenuate Bleomycin-Induced Pulmonary Fibrosis Trough Antioxidant, Anti-Inflammatory and Protease Activity in a Mouse Model
| العنوان: | Zingiber officinale-Derived Extracellular Vesicles Attenuate Bleomycin-Induced Pulmonary Fibrosis Trough Antioxidant, Anti-Inflammatory and Protease Activity in a Mouse Model |
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| المؤلفون: | Alma Aurora Ramírez-Hernández, Edilburga Reyes-Jiménez, Juan Manuel Velázquez-Enríquez, Jovito Cesar Santos-Álvarez, Adriana Soto-Guzmán, Luis Castro-Sánchez, Gabriela Tapia-Pastrana, Honorio Torres-Aguilar, Verónica Rocío Vásquez-Garzón, Rafael Baltiérrez-Hoyos |
| المصدر: | Cells, Vol 12, Iss 14, p 1852 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Cytology |
| مصطلحات موضوعية: | protease activity, SOD, planta extracellular vesicles, antioxidants, idiopathic pulmonary fibrosis, lung inflammation, Cytology, QH573-671 |
| الوصف: | Idiopathic pulmonary fibrosis (IPF) is the most frequent and severe idiopathic interstitial pneumonia. It is a chronic and progressive disease with a poor prognosis and is a major cause of morbidity and mortality. This disease has no cure; therefore, there is a clinical need to search for alternative treatments with greater efficacy. In this study, we aimed to evaluate the effect of extracellular vesicles (EVs) from Zingiber officinale (EVZO) in a murine model of bleomycin (BLM)-induced IPF administered through an osmotic minipump. EVZO had an average size of 373 nm and a spherical morphology, as identified by scanning electron microscopy. Label-free proteomic analysis of EVZOs was performed by liquid chromatography coupled to mass spectrometry, and 20 proteins were identified. In addition, we demonstrated the protease activity of EVZO by gelatin-degrading zymography assay and the superoxide dismutase (SOD) activity of EVZO by an enzymatic assay. In the BLM-induced IPF mouse model, nasal administration of 50 μg of EVZO induced recovery of alveolar space size and decreased cellular infiltrate, collagen deposition, and expression of α-SMA-positive cells. Additionally, EVZO inhibited inflammatory markers such as iNOS and COX-2, lipid peroxidation, and apoptotic cells. These results show that EVZO may represent a novel natural delivery mechanism to treat IPF. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 12141852 2073-4409 |
| Relation: | https://www.mdpi.com/2073-4409/12/14/1852; https://doaj.org/toc/2073-4409 |
| DOI: | 10.3390/cells12141852 |
| URL الوصول: | https://doaj.org/article/5ba32c2697ae4419938c96ce7f397f5e |
| رقم الانضمام: | edsdoj.5ba32c2697ae4419938c96ce7f397f5e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 12141852 20734409 |
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| DOI: | 10.3390/cells12141852 |