Academic Journal
The effect of manipulating glucuronic acid biosynthetic pathway in Bacillus subtilis strain on hyaluronic acid production
| العنوان: | The effect of manipulating glucuronic acid biosynthetic pathway in Bacillus subtilis strain on hyaluronic acid production |
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| المؤلفون: | Shadi Afrasiabi, Fatemeh Sadat Amjad Zanjani, Gholamreza Ahmadian, Reza Ahangari Cohan, Malihe Keramati |
| المصدر: | AMB Express, Vol 13, Iss 1, Pp 1-12 (2023) |
| بيانات النشر: | SpringerOpen, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biotechnology LCC:Microbiology |
| مصطلحات موضوعية: | Biosynthetic pathway genes, Glucuronic acid, Hyaluronic acid, Metabolic engineering, Recombinant Bacillus subtilis, Biotechnology, TP248.13-248.65, Microbiology, QR1-502 |
| الوصف: | Abstract Hyaluronic acid (HA), composed of glucuronic acid (GlcUA) and N-acetyl glucoseamine (GlcNAc), is a versatile biopolymer with high commercial value and innumerous physiological roles and pharmaceutical applications. The hasA gene has main role in HA biosynthesis by Streptococcus strain as a natural producer. The hasB and hasC genes are also mediate GlcUA precursor biosynthesis. In the present study, S. equisimilis hasA gene; B. subtilis tuaD and gtaB genes for GlcUA precursors enhancement, and vgb gene coding bacterial hemoglobin as an oxygen provider were used to construct the B. subtilis strain for HA production. RBSHA (hasA), RBSHA2 (hasA/tuaD/gtaB), and RBSHA3 (hasA/tuaD/gtaB/vgb) strains were developed and confirmed through genotype and phenotype analysis. After HA production and purification, FTIR spectroscopy confirmed the produced HA structures. HA assay showed the highest HA titer for RBSHA3 (2.1 ± 0.18 mg/ml) and then RBSHA2 (1.9 ± 0.03 mg/ml), and RBSHA (0.6 ± 0.14 mg/ml). Statistical analysis indicated there is no significant difference in HA titer between RBSHA2 and RBSHA3 strains (p-value > 0.05), however, these strains produced HA approximately 4-fold higher than that of RBSHA strain. Agarose gel electrophoresis showed the same molecular weight ( |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2191-0855 |
| Relation: | https://doaj.org/toc/2191-0855 |
| DOI: | 10.1186/s13568-023-01567-2 |
| URL الوصول: | https://doaj.org/article/5aec9ddb8df84ba58f9ac01d19f01028 |
| رقم الانضمام: | edsdoj.5aec9ddb8df84ba58f9ac01d19f01028 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 21910855 |
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| DOI: | 10.1186/s13568-023-01567-2 |