Academic Journal

The Addition of Opuntia ficus-indica Ethanolic Extract to a Skimmed Milk-Based Extender Impacts Ram Sperm Quality

التفاصيل البيبلوغرافية
العنوان: The Addition of Opuntia ficus-indica Ethanolic Extract to a Skimmed Milk-Based Extender Impacts Ram Sperm Quality
المؤلفون: Larbi Allai, Xavier Druart, Pinar Terzioğlu, Noureddine Louanjli, Boubker Nasser, Mehmet Ozturk, Bouchra El Amiri
المصدر: Veterinary Medicine International, Vol 2023 (2023)
بيانات النشر: Wiley, 2023.
سنة النشر: 2023
المجموعة: LCC:Veterinary medicine
مصطلحات موضوعية: Veterinary medicine, SF600-1100
الوصف: Recently, researchers have focused on the use of natural antioxidants to improve semen quality as a key element for successful artificial insemination. In this context, the first aim of this study was to determine the antioxidant activity and composition (minerals, vitamins, and sugars) of Opuntia ficus-indica cladode ethanolic extract (ETHEX). A further purpose of the study was to investigate the effect of ETHEX supplementation on the quality of liquid ram semen extended with skim milk (SM) at 5°C. The antioxidant activity of ETHEX was studied using free radical 1, 1-diphenyl-2-picrylhydrazyl (DPPH•) assay. The mineral composition and the sugar and vitamin contents of ETHEX were determined using an inductively coupled plasma optical emission spectrometry (ICP-OES) and HPLC-DAD-RID analytical instruments. As a second part, semen was collected from five Boujaâd rams with an artificial vagina. The ejaculates with more than 70% motility were pooled, extended with skim milk (SM) extender without (control) or supplemented with 1–8% of ETHEX (37°C; 0.8 × 109 sperm/mL). Sperm quality parameters were assessed at 8, 24, 48, and 72 h. The results showed that ETHEX had a higher antioxidant activity compared to those of ascorbic acid and butylated hydroxytoluene (BHT). Furthermore, ETHEX contains a considerable amount of minerals, vitamins, and sugars. The inclusion of 1 or 2% ETHEX in SM increased the sperm motility, viability, and membrane integrity and decreased the abnormality of spontaneous and catalyzed lipids peroxidation (p
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2042-0048
Relation: https://doaj.org/toc/2042-0048
DOI: 10.1155/2023/6248890
URL الوصول: https://doaj.org/article/59b806a19b3e4b51bad77f79c1a21349
رقم الانضمام: edsdoj.59b806a19b3e4b51bad77f79c1a21349
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20420048
DOI:10.1155/2023/6248890