Academic Journal
TAAR8 Mediates Increased Migrasome Formation by Cadaverine in RPE Cells
| العنوان: | TAAR8 Mediates Increased Migrasome Formation by Cadaverine in RPE Cells |
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| المؤلفون: | Joon Bum Kim, Ji-Eun Bae, Na Yeon Park, Yong Hwan Kim, Seong Hyun Kim, Hyejin Hyung, Eunbyul Yeom, Dong Kyu Choi, Kwiwan Jeong, Dong-Hyung Cho |
| المصدر: | Current Issues in Molecular Biology, Vol 46, Iss 8, Pp 8658-8664 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | migrasome, cadaverine, TAAR8, TSPAN4, RPE cells, Biology (General), QH301-705.5 |
| الوصف: | Migrasomes, the newly discovered cellular organelles that form large vesicle-like structures on the retraction fibers of migrating cells, are thought to be involved in communication between neighboring cells, cellular content transfer, unwanted material shedding, and information integration. Although their formation has been described previously, the molecular mechanisms of migrasome biogenesis are largely unknown. Here, we developed a cell line that overexpresses GFP-tetraspanin4, enabling observation of migrasomes. To identify compounds that regulate migrasome activity in retinal pigment epithelial (RPE) cells, we screened a fecal chemical library and identified cadaverine, a biogenic amine, as a potent migrasome formation inducer. Compared with normal migrating cells, those treated with cadaverine had significantly more migrasomes. Putrescine, another biogenic amine, also increased migrasome formation. Trace amine-associated receptor 8 (TAAR8) depletion inhibited migrasome increase in cadaverine-treated RPE cells, and cadaverine also inhibited protein kinase A phosphorylation. In RPE cells, cadaverine triggers migrasome formation via a TAAR8-mediated protein kinase A signaling pathway. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1467-3045 1467-3037 |
| Relation: | https://www.mdpi.com/1467-3045/46/8/510; https://doaj.org/toc/1467-3037; https://doaj.org/toc/1467-3045 |
| DOI: | 10.3390/cimb46080510 |
| URL الوصول: | https://doaj.org/article/580b3e78cf954cfeb2fa64c82ffb38a4 |
| رقم الانضمام: | edsdoj.580b3e78cf954cfeb2fa64c82ffb38a4 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14673045 14673037 |
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| DOI: | 10.3390/cimb46080510 |