Academic Journal
Microarray dataset supporting a role for ATF4 in isoginkgetin-induced gene expression in HCT116 cells.
| العنوان: | Microarray dataset supporting a role for ATF4 in isoginkgetin-induced gene expression in HCT116 cells. |
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| المؤلفون: | Erin van Zyl, Victoria Tolls, Bruce C McKay |
| المصدر: | Data in Brief, Vol 42, Iss , Pp 108126- (2022) |
| بيانات النشر: | Elsevier, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Computer applications to medicine. Medical informatics LCC:Science (General) |
| مصطلحات موضوعية: | ATF4, Isoginkgetin, Microarray, Spliceosome inhibition, Computer applications to medicine. Medical informatics, R858-859.7, Science (General), Q1-390 |
| الوصف: | Isoginkgetin (IGG) is a compound originally derived from the leaves of Ginkgo biloba trees. It was subsequently identified through a chemical screen to be an inhibitor of both the major and minor spliceosome, with an IC50 value of 30 µM [1]. Little is currently known about the overall effects of spliceosome inhibition on human cells. Here, we treated HCT116 and a p53 null subline of colon cancer cells with 30 µM IGG for 8 hours. Total RNA was isolated, and Affymetrix oligonucleotide microarray analysis was completed using samples from two biologically independent experiments. A relatively small number of transcripts were differentially expressed in these cell lines. There was considerable overlap in the upregulated but not the downregulated transcripts. PANTHER Reactome analysis of these shared upregulated transcripts identified enriched pathways involving the ATF4 transcription factor important in the integrated stress response [2]. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2352-3409 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2352340922003365; https://doaj.org/toc/2352-3409 |
| DOI: | 10.1016/j.dib.2022.108126 |
| URL الوصول: | https://doaj.org/article/5793fca4667249e08ac76f4778abef03 |
| رقم الانضمام: | edsdoj.5793fca4667249e08ac76f4778abef03 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 23523409 |
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| DOI: | 10.1016/j.dib.2022.108126 |