Academic Journal
Decreased TRPM7 alleviates high glucose-induced renal tubular epithelial cell injury by inhibiting the HMGB1/TLR4 signaling pathway
| العنوان: | Decreased TRPM7 alleviates high glucose-induced renal tubular epithelial cell injury by inhibiting the HMGB1/TLR4 signaling pathway |
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| المؤلفون: | Wei Feng, Zheng-Yong Cao, Fu-Min Guan, Hong Chen |
| المصدر: | Asian Pacific Journal of Tropical Biomedicine, Vol 13, Iss 9, Pp 393-402 (2023) |
| بيانات النشر: | Wolters Kluwer Medknow Publications, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Arctic medicine. Tropical medicine LCC:Biology (General) |
| مصطلحات موضوعية: | diabetic nephropathy, trpm7, hmgb1/tlr4, high glucose, renal tubular epithelial cell, Arctic medicine. Tropical medicine, RC955-962, Biology (General), QH301-705.5 |
| الوصف: | Objective: To explore the regulatory mechanism of transient receptor potential melastatin-7 (TRPM7) in high glucose-induced renal tubular epithelial cell injury. Methods: The expression of TRPM7 in the serum of diabetic nephropathy patients and high glucose-induced HK-2 cells was detected by RT-qPCR. Then, the TRPM7 interference vector was constructed, and the downstream high mobility group box 1 (HMGB1)/Toll-like receptor 4 (TLR4) signaling pathway proteins were detected. Next, in addition to interference with TRPM7 expression, overexpression of HMGB1 in high glucose-induced HK-2 cells was performed. Cell activity, apoptosis, oxidative stress levels, and inflammation levels were determined by CCK8, TUNEL, Western blotting, immunofluorescence and related kits. Results: TRPM7 expression was upregulated in the serum of diabetic nephropathy patients and high glucose-induced HK-2 cells. Interference with TRPM7 reduced cell damage, epithelial-mesenchymal transition, oxidative stress, and inflammatory response in high glucose-induced HK-2 cells via inhibiting the HMGB1/TLR4 signaling pathway. However, the effects induced by TRPM7 silencing were abrogated by HMGB1 overexpression. Conclusions: Decreased TRPM7 alleviates high glucose-induced renal tubular epithelial cell injury by inhibiting the HMGB1/TLR4 signaling pathway. Further animal experiments and clinical trials are warranted to verify its effect. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2221-1691 2588-9222 |
| Relation: | http://www.apjtb.org/article.asp?issn=2221-1691;year=2023;volume=13;issue=9;spage=393;epage=402;aulast=Feng; https://doaj.org/toc/2221-1691; https://doaj.org/toc/2588-9222 |
| DOI: | 10.4103/2221-1691.385570 |
| URL الوصول: | https://doaj.org/article/573029f0c1e94e05a6b336dab7f1c5e3 |
| رقم الانضمام: | edsdoj.573029f0c1e94e05a6b336dab7f1c5e3 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 22211691 25889222 |
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| DOI: | 10.4103/2221-1691.385570 |