Academic Journal
Short-hairpin RNA-mediated suppression of cortactin may inhibit the migration and invasion abilities of endometrial cancer cells by reducing lamellipodia
| العنوان: | Short-hairpin RNA-mediated suppression of cortactin may inhibit the migration and invasion abilities of endometrial cancer cells by reducing lamellipodia |
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| المؤلفون: | Huisi Lin, Yujuan Fan, Zhifu Zhi, Lihong Pang, Dan Sun |
| المصدر: | Iranian Journal of Basic Medical Sciences, Vol 26, Iss 12, Pp 1390-1399 (2023) |
| بيانات النشر: | Mashhad University of Medical Sciences, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | cell migration, cortactin, endometrial neoplasms, pseudopodia, rac1, Medicine |
| الوصف: | Objective(s): The prognosis of endometrial cancer (EC) is significantly affected by tumor infiltration and metastasis. Cortactin (CTTN) regulates infiltration and metastasis in other tumors. Studies on the role and mechanism of CTTN in EC are limited and further studies are needed.Materials and Methods: Quantitative PCR and immunohistochemistry were used to detect Ras-associated C3 botulinum toxin substrate 1 (Rac1) and CTTN in EC and normal tissues. The relationship between the expression of these two genes and their prognostic factors was analyzed. A CTTN-RNAi lentiviral system was constructed and transfected into EC cells. Migration and invasion were evaluated by scratch assay, transwell migration, and invasion assays. Pseudopodia formation was observed by immunofluorescence staining. Western blotting was performed to detect the expression of Rac1.Results: The expression levels of Rac1 and CTTN in EC tissues were significantly higher than those in normal tissues. In the EC group, Rac1 and CTTN levels were correlated. The protein expression levels of Rac1 and CTTN were related to myometrial invasion and stage. After CTTN knockdown, the migration rate, invasiveness, and migratory ability of EC cells decreased significantly. Lamellipodia was observed to disappear with the appearance of blebs. Rac1 protein expression was decreased after CTTN knockdown.Conclusion: CTTN may promote the invasion and migration of EC by lamellipodia. This effect may be related to the regulation of Rac1 by CTTN. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2008-3866 2008-3874 |
| Relation: | https://ijbms.mums.ac.ir/article_22790_35c31d274b72c74efcc0c70ff016c02a.pdf; https://doaj.org/toc/2008-3866; https://doaj.org/toc/2008-3874 |
| DOI: | 10.22038/ijbms.2023.67633.14863 |
| URL الوصول: | https://doaj.org/article/c555ca79a4434fbdbb43947eddac0150 |
| رقم الانضمام: | edsdoj.555ca79a4434fbdbb43947eddac0150 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20083866 20083874 |
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| DOI: | 10.22038/ijbms.2023.67633.14863 |