التفاصيل البيبلوغرافية
| العنوان: |
Lactoferrin-modified nanoemulsions enhance brain-targeting and therapeutic efficacy of arctigenin against Toxoplasma gondii-induced neuronal injury |
| المؤلفون: |
Jing-Mei Lu, Guang-Nan Jin, Yan Xin, Jing-Wen Ma, Xin-Yu Shen, Yan-Zhu Quan, Yi-Ming Liu, Jin-Yi Zhou, Bing-Zhe Wang, Ying-Biao Li, Xiang Xu, Lian-Xun Piao |
| المصدر: |
International Journal for Parasitology: Drugs and Drug Resistance, Vol 27, Iss , Pp 100575- (2025) |
| بيانات النشر: |
Elsevier, 2025. |
| سنة النشر: |
2025 |
| المجموعة: |
LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: |
Arctigenin, Lactoferrin, Nanoemulsions, Toxoplasma gondii, Neuron, Microglia, Infectious and parasitic diseases, RC109-216 |
| الوصف: |
Toxoplasma gondii, a neurotropic protozoan parasite, affects the central nervous system and causes various neurological disorders. Previous studies have demonstrated that Arctigenin (AG) exhibits anti-T. gondii activity and reduces depression-like behaviors induced by T. gondii infection. This study aimed to enhance AG's brain-targeting and therapeutic efficacy by developing lactoferrin-modified nanoemulsions loaded with AG (Lf-AG-NEs). Lf-modified nanoemulsions were prepared and assessed using in vivo and in vitro infection models with the T. gondii RH strain, and a co-culture system of BV2 microglia and primary neuron cells. The effects of Lf-AG-NEs on T. gondii-induced neuronal injury were examined, and potential molecular mechanisms were elucidated through real-time quantitative PCR, western blotting, immunofluorescence, flow cytometry, immunohistochemistry, and Nissl staining. In vitro assessments showed significant increases in cellular uptake and blood-brain barrier penetration by Lf-AG-NEs. These nanoemulsions notably inhibited T. gondii proliferation in brain tissue and BV2 cells, surpassing the effects of free AG or AG-NEs alone. Additionally, Lf-AG-NEs substantially alleviated neuropathological changes and reduced microglial activation and neuroinflammation by downregulating the TLR4/NF-κB and TNFR1/NF-κB signaling pathways. Co-culturing BV2 cells with primary cortical neurons indicated that Lf-AG-NEs, similarly to CLI-095 and R7050, attenuated T. gondii-induced microglial activation and subsequent neuronal injury. In conclusion, the successfully prepared Lf-AG-NEs not only enhanced the anti-T. gondii effect but also strengthened the protective impact against neuronal injury induced by T. gondii, through the modulation of microglial signaling pathways. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2211-3207 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2211320724000563; https://doaj.org/toc/2211-3207 |
| DOI: |
10.1016/j.ijpddr.2024.100575 |
| URL الوصول: |
https://doaj.org/article/edc553f0978f4e4d8aff7d34b5f6c69d |
| رقم الانضمام: |
edsdoj.553f0978f4e4d8aff7d34b5f6c69d |
| قاعدة البيانات: |
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