Academic Journal
A lipopeptide facilitate induction of Mycobacterium leprae killing in host cells.
| العنوان: | A lipopeptide facilitate induction of Mycobacterium leprae killing in host cells. |
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| المؤلفون: | Yumi Maeda, Toshiki Tamura, Yasuo Fukutomi, Tetsu Mukai, Masanori Kai, Masahiko Makino |
| المصدر: | PLoS Neglected Tropical Diseases, Vol 5, Iss 11, p e1401 (2011) |
| بيانات النشر: | Public Library of Science (PLoS), 2011. |
| سنة النشر: | 2011 |
| المجموعة: | LCC:Arctic medicine. Tropical medicine LCC:Public aspects of medicine |
| مصطلحات موضوعية: | Arctic medicine. Tropical medicine, RC955-962, Public aspects of medicine, RA1-1270 |
| الوصف: | Little is known of the direct microbicidal activity of T cells in leprosy, so a lipopeptide consisting of the N-terminal 13 amino acids lipopeptide (LipoK) of a 33-kD lipoprotein of Mycobacterium leprae, was synthesized. LipoK activated M. leprae infected human dendritic cells (DCs) to induce the production of IL-12. These activated DCs stimulated autologous CD4+ or CD8+ T cells towards type 1 immune response by inducing interferon-gamma secretion. T cell proliferation was also evident from the CFSE labeling of target CD4+ or CD8+ T cells. The direct microbicidal activity of T cells in the control of M. leprae multiplication is not well understood. The present study showed significant production of granulysin, granzyme B and perforin from these activated CD4+ and CD8+ T cells when stimulated with LipoK activated, M. leprae infected DCs. Assessment of the viability of M. leprae in DCs indicated LipoK mediated T cell-dependent killing of M. leprae. Remarkably, granulysin as well as granzyme B could directly kill M. leprae in vitro. Our results provide evidence that LipoK could facilitate M. leprae killing through the production of effector molecules granulysin and granzyme B in T cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1935-2735 |
| Relation: | http://europepmc.org/articles/PMC3222628?pdf=render; https://doaj.org/toc/1935-2735 |
| DOI: | 10.1371/journal.pntd.0001401 |
| URL الوصول: | https://doaj.org/article/54b80051cdb74e6db95a50dfcc07424d |
| رقم الانضمام: | edsdoj.54b80051cdb74e6db95a50dfcc07424d |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19352735 |
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| DOI: | 10.1371/journal.pntd.0001401 |