Academic Journal
Bacterial lipopolysaccharide model of neuroinflammation-associated neurodegeneration in Wistar rats: A comparison between different durations of lipopolysaccharide induction
| العنوان: | Bacterial lipopolysaccharide model of neuroinflammation-associated neurodegeneration in Wistar rats: A comparison between different durations of lipopolysaccharide induction |
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| المؤلفون: | Vandana Blossom, Sheetal Dinkar Ullal, Rajalakshmi Rai, Shrijeet Chakraborthi, Nayanatara Arun Kumar, Mangala M. Pai, Rajanigandha Vadgaonkar |
| المصدر: | Veterinary World, Vol 17, Iss 11, Pp 2567-2576 (2024) |
| بيانات النشر: | Veterinary World, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Animal culture LCC:Veterinary medicine |
| مصطلحات موضوعية: | astrocyte, lipopolysaccharide, mental health, microglia, neurodegeneration, neuroinflammation, Animal culture, SF1-1100, Veterinary medicine, SF600-1100 |
| الوصف: | Background and Aim: Bacterial lipopolysaccharide (LPS)-induced neuroinflammation can be the most dependable animal model for studying neurodegeneration mechanisms driven by systemic inflammation-induced neuroinflammation. Hence, this study aimed to standardize the LPS model of neuroinflammation by comparing the effect of relatively low-dose LPS administered for different durations on the induction of neurodegeneration in Wistar rats. Materials and Methods: Six groups of six adult Wistar rats per group were used in the study. Group 1 was the control group, and the other five were administered single weekly dose of LPS (170 μg/kg) for increasing durations, ranging from 4 weeks to 8 weeks. The study endpoints included behavioral parameters, neuronal assay results, and the expression of microglia and astrocytes in the frontal cortex, dentate gyrus, and hippocampus. Results: We observed a significant reduction in the number of neurons and an increase in glial cells at 5 weeks of exposure, along with a decline in memory. Thereafter, these changes were gradual until 7 weeks of exposure. However, at 8 weeks of exposure, there was no further statistically significant worsening compared with the group exposed for 7 weeks. Conclusion: To effectively induce neuroinflammation and cause neuronal damage, a minimum of five weekly LPS administrations at a dose of 170 μg/kg is required. Moreover, our results recommend a maximum of 7 weeks of LPS exposure to create a chronic inflammatory model of neuroinflammation. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2567-2576 0972-8988 2231-0916 |
| Relation: | https://www.veterinaryworld.org/Vol.17/November-2024/16.pdf; https://doaj.org/toc/0972-8988; https://doaj.org/toc/2231-0916 |
| DOI: | 10.14202/vetworld.2024.2567-2576 |
| URL الوصول: | https://doaj.org/article/c53edc4fbd2a40bd8624729266a66451 |
| رقم الانضمام: | edsdoj.53edc4fbd2a40bd8624729266a66451 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 25672576 09728988 22310916 |
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| DOI: | 10.14202/vetworld.2024.2567-2576 |