التفاصيل البيبلوغرافية
| العنوان: |
16S rRNA Gene Sequencing for Deciphering the Colorectal Cancer Gut Microbiome: Current Protocols and Workflows |
| المؤلفون: |
Muhammad-Afiq Osman, Hui-min Neoh, Nurul-Syakima Ab Mutalib, Siok-Fong Chin, Rahman Jamal |
| المصدر: |
Frontiers in Microbiology, Vol 9 (2018) |
| بيانات النشر: |
Frontiers Media S.A., 2018. |
| سنة النشر: |
2018 |
| المجموعة: |
LCC:Microbiology |
| مصطلحات موضوعية: |
gut microbiome, metagenomics, next-generation sequencing, 16S rRNA gene, 16S rRNA gene sequencing, colorectal cancer, Microbiology, QR1-502 |
| الوصف: |
The human gut holds the densest microbiome ecosystem essential in maintaining a healthy host physiology, whereby disruption of this ecosystem has been linked to the development of colorectal cancer (CRC). The advent of next-generation sequencing technologies such as the 16S rRNA gene sequencing has enabled characterization of the CRC gut microbiome architecture in an affordable and culture-free approach. Nevertheless, the lack of standardization in handling and storage of biospecimens, nucleic acid extraction, 16S rRNA gene primer selection, length, and depth of sequencing and bioinformatics analyses have contributed to discrepancies found in various published studies of this field. Accurate characterization of the CRC microbiome found in different stages of CRC has the potential to be developed into a screening tool in the clinical setting. This mini review aims to concisely compile all available CRC microbiome studies performed till end of 2016 and to suggest standardized protocols that are crucial in developing a gut microbiome screening panel for CRC. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1664-302X |
| Relation: |
http://journal.frontiersin.org/article/10.3389/fmicb.2018.00767/full; https://doaj.org/toc/1664-302X |
| DOI: |
10.3389/fmicb.2018.00767 |
| URL الوصول: |
https://doaj.org/article/53c71c7f43354d1a98c63ffe371fdb71 |
| رقم الانضمام: |
edsdoj.53c71c7f43354d1a98c63ffe371fdb71 |
| قاعدة البيانات: |
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