التفاصيل البيبلوغرافية
| العنوان: |
Enhancing the detection of Toxoplasma gondii via an anti-SAG1 scFv-alkaline phosphatase immunoconjugate |
| المؤلفون: |
Emna Hannachi, Aïda Bouratbine, Mohamed Mousli |
| المصدر: |
Biotechnology Reports, Vol 23, Iss , Pp - (2019) |
| بيانات النشر: |
Elsevier, 2019. |
| سنة النشر: |
2019 |
| المجموعة: |
LCC:Biotechnology |
| مصطلحات موضوعية: |
Biotechnology, TP248.13-248.65 |
| الوصف: |
The purpose of this study was to implement a fluorometric method for enhancing the detection sensitivity of Toxoplasma gondii in biological fluids. To address this challenge, we designed and produced a recombinant immunoconjugate tool based on a single-chain antibody fragment anti-T. gondii SAG1 antigen (scFvSG15) genetically fused to the bacterial alkaline phosphatase (AP) using 4-methyl-umbelliferyl-phosphate as fluorogenic substrate. The anti-SAG1 scFv-AP conjugate was fully bifunctional and was used successfully in different assays including immunoblot, fluorometric ELISA and direct immunofluorescence. The fluorometric immunoassay afforded an extremely low detection limit (1 tachyzoite/well), which was in agreement with the real-time PCR control test. The immunofluorescence imaging has provided captivating visual evidence of T. gondii detection. These results strongly suggest that the recombinant anti-SAG1-AP conjugate generated here might serve as useful and highly sensitive immunoassay probe to direct detect T. gondii in a one-step procedure, opening up new perspectives for diagnosis of toxoplasmosis. Keywords: Alkaline phosphatase, Fluorescent immunodetection, Recombinant conjugate, Single-chain antibody fragment variable (scFv), Toxoplasma gondii |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2215-017X |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2215017X19302474; https://doaj.org/toc/2215-017X |
| DOI: |
10.1016/j.btre.2019.e00360 |
| URL الوصول: |
https://doaj.org/article/53636698cfa44446b87351c861a557ea |
| رقم الانضمام: |
edsdoj.53636698cfa44446b87351c861a557ea |
| قاعدة البيانات: |
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