Academic Journal
Investigation of the impact of thirdhand e-cigarette exposure on platelet function: A pre-clinical study
| العنوان: | Investigation of the impact of thirdhand e-cigarette exposure on platelet function: A pre-clinical study |
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| المؤلفون: | Shelby S. Umphres, Ahmed B. Alarabi, Hamdy E. A. Ali, Fadi T. Khasawneh, Fatima Z. Z. Alshbool |
| المصدر: | Tobacco Induced Diseases, Vol 22, Iss March, Pp 1-10 (2024) |
| بيانات النشر: | European Publishing, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Diseases of the respiratory system LCC:Neoplasms. Tumors. Oncology. Including cancer and carcinogens |
| مصطلحات موضوعية: | e-cigarette, platelet, thrombosis, thirdhand tobacco exposure, cardiovascular disease, Diseases of the respiratory system, RC705-779, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| الوصف: | Introduction The use of e-cigarettes (ECs) has reached unprecedented levels, due to a variety of reasons, including the misconception regarding their safety. Thus, there have been efforts to characterize the effects of EC exposure, including in the context of thirdhand EC (THEC) on a host of disorders, such as cardiovascular disease (CVD). Methods To address this issue, we sought to characterize the effects of THEC on platelet function and thrombus formation, using a novel mouse exposure protocol that resembles real life scenarios. To assess these effects, a host of related in vivo (i.e. tail bleeding time, and ferric chloride injury induced thrombosis model) assays and in vitro platelet specific (e.g. aggregation, and dense granule secretion) investigative assays were conducted. Results Our in vivo characterization demonstrated that THEC exposed mice exhibited a prothrombotic phenotype reflected by their shortened tail bleeding (THEC: 37 ± 15 seconds, versus clean air: 183 ± 56 s) and occlusion times (THEC: 188 ± 39 s, versus clean air: 519 ± 70 s), relative to those exposed to clean air. Importantly, we found no difference in the platelet counts between the THEC and clean air mice. As for the underlying mechanism, separate experiments revealed significantly enhanced platelet aggregation, dense and alpha granule secretion, as well as integrin/GPIIb-IIIa activation and phosphatidylserine exposure in response to thrombin and ADP agonist stimulation. Conclusions Taken together, these results provide evidence that THEC does have the capacity to increase the risk of thrombotic disease, which should increase awareness regarding its underappreciated negative health effects. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1617-9625 |
| Relation: | https://www.tobaccoinduceddiseases.org/Investigation-of-the-impact-of-thirdhand-e-cigarette-exposure-non-platelet-function,185286,0,2.html; https://doaj.org/toc/1617-9625 |
| DOI: | 10.18332/tid/185286 |
| URL الوصول: | https://doaj.org/article/c524a02b58cb4c1a80b887e375498eef |
| رقم الانضمام: | edsdoj.524a02b58cb4c1a80b887e375498eef |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16179625 |
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| DOI: | 10.18332/tid/185286 |