التفاصيل البيبلوغرافية
| العنوان: |
Structural Insights into the Rrp4 Subunit from the Crystal Structure of the Thermoplasma acidophilum Exosome |
| المؤلفون: |
Seonha Park, Hyun Sook Kim, Kyuhyeon Bang, Ahreum Han, Byeongmin Shin, Minjeong Seo, Sulhee Kim, Kwang Yeon Hwang |
| المصدر: |
Biomolecules, Vol 14, Iss 6, p 621 (2024) |
| بيانات النشر: |
MDPI AG, 2024. |
| سنة النشر: |
2024 |
| المجموعة: |
LCC:Microbiology |
| مصطلحات موضوعية: |
exosome, RNase, PNPase, RNA processing, quality control, Microbiology, QR1-502 |
| الوصف: |
The exosome multiprotein complex plays a critical role in RNA processing and degradation. This system governs the regulation of mRNA quality, degradation in the cytoplasm, the processing of short noncoding RNA, and the breakdown of RNA fragments. We determined two crystal structures of exosome components from Thermoplasma acidophilum (Taci): one with a resolution of 2.3 Å that reveals the central components (TaciRrp41 and TaciRrp42), and another with a resolution of 3.5 Å that displays the whole exosome (TaciRrp41, TaciRrp42, and TaciRrp4). The fundamental exosome structure revealed the presence of a heterodimeric complex consisting of TaciRrp41 and TaciRrp42. The structure comprises nine subunits, with TaciRrp41 and TaciRrp42 arranged in a circular configuration, while TaciRrp4 is located at the apex. The RNA degradation capabilities of the TaciRrp4:41:42 complex were verified by RNA degradation assays, consistent with prior findings in other archaeal exosomes. The resemblance between archaeal exosomes and bacterial PNPase suggests a common mechanism for RNA degradation. Despite sharing comparable topologies, the surface charge distributions of TaciRrp4 and other archaea structures are surprisingly distinct. Different RNA breakdown substrates may be responsible for this variation. These newfound structural findings enhance our comprehension of RNA processing and degradation in biological systems. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2218-273X |
| Relation: |
https://www.mdpi.com/2218-273X/14/6/621; https://doaj.org/toc/2218-273X |
| DOI: |
10.3390/biom14060621 |
| URL الوصول: |
https://doaj.org/article/dd5118c8f98f476cbcad14202c5b2e9b |
| رقم الانضمام: |
edsdoj.5118c8f98f476cbcad14202c5b2e9b |
| قاعدة البيانات: |
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