التفاصيل البيبلوغرافية
| العنوان: |
Rapid detection of SARS-CoV-2 variants by molecular-clamping technology-based RT-qPCR |
| المؤلفون: |
Shuo Shen, Andrew Y. Fu, Maidar Jamba, Jonathan Li, Zhen Cui, Larry Pastor, Daniel Cataldi, Qing Sun, Joseph A. Pathakamuri, Daniel Kuebler, Michael Rohall, Madison Krohn, Daniel Kissinger, Jocelyn Neves, Isaac Archibeque, Aiguo Zhang, Chuanyi M. Lu, Michael Y. Sha |
| المصدر: |
Microbiology Spectrum, Vol 12, Iss 11 (2024) |
| بيانات النشر: |
American Society for Microbiology, 2024. |
| سنة النشر: |
2024 |
| المجموعة: |
LCC:Microbiology |
| مصطلحات موضوعية: |
SARS-CoV-2 variant detection, molecular-clamping technology, XNA, Microbiology, QR1-502 |
| الوصف: |
ABSTRACT Given the challenges that SARS-CoV-2 variants have caused in terms of rapid spread and reduced vaccine efficacy, a rapid and cost-effective assay that can detect new and emerging variants is greatly needed worldwide. We have successfully applied the xenonucleic acid-based molecular-clamping technology to develop a multiplex reverse-transcription quantitative real-time PCR assay for SARS-CoV-2 multivariant detection. The assay was used to test 649 nasopharyngeal swab samples that were collected for clinical diagnosis or surveillance. The assay was able to correctly identify all 36 Delta variant samples as it accurately detected the D614G, T478K, and L452R mutations. In addition, the assay was able to correctly identify all 34 Omicron samples by detecting the K417N, T478K, N501Y, and D614G mutations. This technique reliably detects a variety of variants and has an analytical sensitivity of 100 copies/mL. In conclusion, this novel assay can serve as a rapid and cost-effective tool to facilitate large-scale detection of SARS-CoV-2 variants.IMPORTANCEWe have developed a multiplex reverse-transcription quantitative real-time PCR (RT-qPCR) testing platform for the rapid detection of SARS-CoV-2 variants using the xenonucleic acid (XNA)-based molecular-clamping technology. The XNA-based RT-qPCR assay can achieve high sensitivity with a limit of detection of about 100 copies/mL for variant detection which is much better than the next-generation sequencing (NGS) assay. Its turnaround time is about 4 hours with lower cost and a lot of Clinical Laboratory Improvement Amendments (CLIA) labs own the instrument and meet skillset requirements. This assay provides a rapid, reliable, and cost-effective testing platform for rapid detection and monitoring of known and emerging SARS-CoV-2 variants. This testing platform can be adopted by laboratories that perform routine SARS-CoV-2 PCR testing, providing a rapid and cost-effective method in lieu of NGS-based assays, for detecting, differentiating, and monitoring SARS-CoV-2 variants. This assay is easily scalable to any new variant(s) should it emerge. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2165-0497 |
| Relation: |
https://doaj.org/toc/2165-0497 |
| DOI: |
10.1128/spectrum.04248-23 |
| URL الوصول: |
https://doaj.org/article/4ec10dc128264475a23af032c1f1b9d2 |
| رقم الانضمام: |
edsdoj.4ec10dc128264475a23af032c1f1b9d2 |
| قاعدة البيانات: |
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