التفاصيل البيبلوغرافية
| العنوان: |
Multi-Omics Exploration of the Mechanism of Curcumol to Reduce Invasion and Metastasis of Nasopharyngeal Carcinoma by Inhibiting NCL/EBNA1-Mediated UBE2C Upregulation |
| المؤلفون: |
Haiping Liu, Juan Wang, Lin Wang, Wei Tang, Xinyue Hou, Yi Zhun Zhu, Xu Chen |
| المصدر: |
Biomolecules, Vol 14, Iss 9, p 1142 (2024) |
| بيانات النشر: |
MDPI AG, 2024. |
| سنة النشر: |
2024 |
| المجموعة: |
LCC:Microbiology |
| مصطلحات موضوعية: |
curcumol, transcriptomics, proteomics, metabolomics, nasopharyngeal carcinoma, NCL, Microbiology, QR1-502 |
| الوصف: |
Nasopharyngeal carcinoma (NPC) is closely linked to Epstein–Barr virus (EBV) infection. Curcumae Rhizoma, a traditional Chinese herb, has shown antitumor effects, primarily through its component curcumol (Cur), which has been shown to reduce NPC cell invasion and migration by targeting nucleolin (NCL) and Epstein–Barr Virus Nuclear Antigen 1 (EBNA1). We constructed an EBV-positive NPC cell model using C666-1 cells and performed transcriptomics studies after treatment with curcumol, which revealed a significant enrichment of ubiquitin-mediated proteolysis, the PI3K-AKT and mTOR signaling pathways, cell cycle and apoptosis involved in tumor invasion and migration. To investigate the importance of NCL and EBNA1 in curcumol-resistant EBV-positive NPC, we performed a multi-omics study using short hairpin NCL (shNCL) and shEBNA1 EBV-positive NPC cells, and the proteomics results showed enrichment in complement and coagulation cascades and ubiquitin-mediated proteolysis signaling pathways. Here, we focused on ubiquitin-conjugating enzyme E2C (UBE2C), which plays an important role in the ubiquitin-mediated proteolysis signaling pathway. In addition, metabolomics revealed that UBE2C is highly associated with 4-Aminobutanoic acid (GABA). In vitro studies further validated the function of the key targets, suggesting that UBE2C plays an important role in NCL and EBNA1-mediated curcumol resistance to nasopharyngeal carcinoma invasion and metastasis. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2218-273X |
| Relation: |
https://www.mdpi.com/2218-273X/14/9/1142; https://doaj.org/toc/2218-273X |
| DOI: |
10.3390/biom14091142 |
| URL الوصول: |
https://doaj.org/article/4ea29e981ecb42f7b0f96765fc0a48f4 |
| رقم الانضمام: |
edsdoj.4ea29e981ecb42f7b0f96765fc0a48f4 |
| قاعدة البيانات: |
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