التفاصيل البيبلوغرافية
| العنوان: |
A novel metabarcoding primer pair for environmental DNA analysis of Cephalopoda (Mollusca) targeting the nuclear 18S rRNA region |
| المؤلفون: |
Daniëlle S. W. de Jonge, Véronique Merten, Till Bayer, Oscar Puebla, Thorsten B. H. Reusch, Henk-Jan T. Hoving |
| المصدر: |
Royal Society Open Science, Vol 8, Iss 2 (2021) |
| بيانات النشر: |
The Royal Society, 2021. |
| سنة النشر: |
2021 |
| المجموعة: |
LCC:Science |
| مصطلحات موضوعية: |
metabarcoding, environmental dna, cephalopoda, universal primer, Science |
| الوصف: |
Cephalopods are pivotal components of marine food webs, but biodiversity studies are hampered by challenges to sample these agile marine molluscs. Metabarcoding of environmental DNA (eDNA) is a potentially powerful technique to study oceanic cephalopod biodiversity and distribution but has not been applied thus far. We present a novel universal primer pair for metabarcoding cephalopods from eDNA, Ceph18S (Forward: 5′-CGC GGC GCT ACA TAT TAG AC-3′, Reverse: 5′-GCA CTT AAC CGA CCG TCG AC-3′). The primer pair targets the hypervariable region V2 of the nuclear 18S rRNA gene and amplifies a relatively short target sequence of approximately 200 bp in order to allow the amplification of degraded DNA. In silico tests on a reference database and empirical tests on DNA extracts from cephalopod tissue estimate that 44–66% of cephalopod species, corresponding to about 310–460 species, can be amplified and identified with this primer pair. A multi-marker approach with the novel Ceph18S and two previously published cephalopod mitochondrial 16S rRNA primer sets targeting the same region (Jarman et al. 2006 Mol. Ecol. Notes. 6, 268–271; Peters et al. 2015 Mar. Ecol. 36, 1428–1439) is estimated to amplify and identify 89% of all cephalopod species, of which an estimated 19% can only be identified by Ceph18S. All sequences obtained with Ceph18S were submitted to GenBank, resulting in new 18S rRNA sequences for 13 cephalopod taxa. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2054-5703 |
| Relation: |
https://doaj.org/toc/2054-5703 |
| DOI: |
10.1098/rsos.201388 |
| URL الوصول: |
https://doaj.org/article/d4e156eb57f54c23bf7552c68cde2d3e |
| رقم الانضمام: |
edsdoj.4e156eb57f54c23bf7552c68cde2d3e |
| قاعدة البيانات: |
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