Academic Journal
Validation of Fluorescent SSCP Analysis for Sensitive Detection of p53 Mutations
| العنوان: | Validation of Fluorescent SSCP Analysis for Sensitive Detection of p53 Mutations |
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| المؤلفون: | L. Moore, T. Godfrey, C. Eng, A. Smith, R. Ho, F.M. Waldman |
| المصدر: | BioTechniques, Vol 28, Iss 5, Pp 986-992 (2000) |
| بيانات النشر: | Taylor & Francis Group, 2000. |
| سنة النشر: | 2000 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | Biology (General), QH301-705.5 |
| الوصف: | We have developed a fluorescence-based single strand conformation polymorphism (SSCP) method that offers fast and sensitive screening for mutations in exons 5–8 of the human p53 gene. The method uses an ABI 377 DNA sequencer for unique color detection of each strand, plus accurate alignment of lanes for better detection of mobility shifts. To validate the method, 21 cell lines with reported mutations in p53 exons 5–8 were analyzed by SSCP using various gel conditions. The sensitivity for mutation detection was 95% for all cell lines studied, and no false positives were seen in 10 normal DNA samples for all four exons. Experiments mixing known amounts of tumor and normal DNA showed that mutations were detected even when tumor DNA was mixed with 80% normal DNA. Fluorescent SSCP analysis using the ABI sequencer is a useful tool in cancer research, where screening large numbers of samples for p53 mutations is desired. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1940-9818 0736-6205 |
| Relation: | https://doaj.org/toc/0736-6205; https://doaj.org/toc/1940-9818 |
| DOI: | 10.2144/00285rr04 |
| URL الوصول: | https://doaj.org/article/4dee60b1c76e40cd91f104a8a1420628 |
| رقم الانضمام: | edsdoj.4dee60b1c76e40cd91f104a8a1420628 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19409818 07366205 |
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| DOI: | 10.2144/00285rr04 |