Academic Journal
Presence of Escherichia coli O157:H7 in Dairy Farms located in Najaf, Baghdad, Kirkuk, and Erbil, Iraq
| العنوان: | Presence of Escherichia coli O157:H7 in Dairy Farms located in Najaf, Baghdad, Kirkuk, and Erbil, Iraq |
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| المؤلفون: | F Najd Obaid, K. A Al-Dabbagh, Y Salam Karim, O Abdallah Mahdi, A Mahdi Rheima, O. K. A. Alkadir, D. A Hamad, E Mahmood Shihab, N Hussin Alwan, E. R Alwaily |
| المصدر: | Archives of Razi Institute, Vol 77, Iss 3, Pp 1261-1265 (2022) |
| بيانات النشر: | Razi Vaccine and Serum Research Institute, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Veterinary medicine |
| مصطلحات موضوعية: | milk, escherichia coli o157:h7, polymerase chain reaction, Veterinary medicine, SF600-1100 |
| الوصف: | It has been approved that one of the most dangerous foodborne pathogenic bacteria is E. coli O157:H7, which is responsible for several infection and death cases worldwide. It is well documented that in the developing countries E. coli O157:H7 is considered the main causative pathogen of human gastrointestinal infections. Therefore, the current research was aimed to evaluate the prevalence of E. coli O157:H7 in dairy cattle's milk using a rapid method, in Iraq (Najaf, Baghdad, Kirkuk, and Erbil). Over a period of 6 months (During hot months) samples were obtained and investigated by culturing on selective media (CT-SMAC). The multiplex PCR (m-PCR) also used for milk sample direct investigation. Using biochemical tests the recorded data showed that, 2 recognized isolates were E. coli, while the recorded data obtained from m-PCR assay revealed that none of the isolated E. coli was toxigenic E.coli O157:H7. The results of m-PCR on the milk samples revealed that 45 milk samples contained at least one of the following genes: O157, H7, stx1, stx2 genes. Also the results of the m-PCR revealed that 2 samples (raw milk) were toxigenic O157:H7 positive. In conclusion, to the best of authors' knowledge, this investigation was the first report on the prevalence of E. coli O157:H7 in the raw milk samples in Iraq. The results showed that the proportion of contaminated milk samples contaminated with E. coli O157:H7 identified in the current survey were similar to that the results of the previously published research from different dairy products across different countries in the Middle East region. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English French |
| تدمد: | 0365-3439 2008-9872 |
| Relation: | https://archrazi.areeo.ac.ir/article_126762_2be1c27fc48e35f686e5a48b52148847.pdf; https://doaj.org/toc/0365-3439; https://doaj.org/toc/2008-9872 |
| DOI: | 10.22092/ari.2022.358832.2317 |
| URL الوصول: | https://doaj.org/article/4dc62c2c3f684a37a711d43a3ee7e187 |
| رقم الانضمام: | edsdoj.4dc62c2c3f684a37a711d43a3ee7e187 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 03653439 20089872 |
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| DOI: | 10.22092/ari.2022.358832.2317 |