Academic Journal
Structural elucidation of mulberry leaf oligosaccharide and its selective promotion of gut microbiota to alleviate type 2 diabetes mellitus
| العنوان: | Structural elucidation of mulberry leaf oligosaccharide and its selective promotion of gut microbiota to alleviate type 2 diabetes mellitus |
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| المؤلفون: | Tenggen Hu, Yuanshan Yu, Jijun Wu, Yujuan Xu, Gengsheng Xiao, Kejing An, Erna Li, Sentai Liao, Yuxiao Zou |
| المصدر: | Food Science and Human Wellness, Vol 13, Iss 4, Pp 2161-2173 (2024) |
| بيانات النشر: | Tsinghua University Press, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Nutrition. Foods and food supply |
| مصطلحات موضوعية: | mulberry leaf oligosaccharide, characterization, gut microbiota, lactobacillus murinus, hypoglycemic activity, Nutrition. Foods and food supply, TX341-641 |
| الوصف: | Two oligosaccharide fractions (MLO 2-1 and 2-2) were purified from enzymatic hydrolysate of mulberry leaf polysaccharide. The results of simulated digestion showed that MLO 2-2 was a digestible oligosaccharide, which could be degraded by human digestive juice; while MLO 2-1 possessed the non-digestible property in the upper gastrointestinal tract and performed the function by regulating the gut microbiota. Hence, MLO 2-1 was selected for the further analysis. The structure of MLO 2-1 was elucidated as follow: α-T-Glcp-(1→3)-β-Glcp-(1→5)-α-Araf-(1→5)-α-Araf-1→5)-α-Araf-(1→3)-α-(2-OAc)-Glcp-1. The in vitro fecal fermentation results showed that MLO 2-1 could modulate the composition of gut microbiota. Meanwhile, MLO 2-1 was effectively metabolized by fecal bacteria to produce lactate and short chain fatty acids, especially acetate and butyrate. The specific metabolic pathways of MLO 2-1 by gut microbiota were further illuminated. Gut microbiota analysis revealed that MLO 2-1 selectively promoted the growth of Ligilactobacillus murinus, a commensal bacterium presented a reduced level in T2DM mice. Animal experiments indicated that MLO 2-1 and L. murinus exhibited hypoglycemic activities. These results demonstrated that MLO 2-1 might alleviate T2DM by selectively accelerating the proliferation of L. murinus. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2097-0765 2213-4530 |
| Relation: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250180; https://doaj.org/toc/2097-0765; https://doaj.org/toc/2213-4530 |
| DOI: | 10.26599/FSHW.2022.9250180 |
| URL الوصول: | https://doaj.org/article/d4cc3f28876840f39981493cd38ab3db |
| رقم الانضمام: | edsdoj.4cc3f28876840f39981493cd38ab3db |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20970765 22134530 |
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| DOI: | 10.26599/FSHW.2022.9250180 |