Academic Journal
Estudo comparativo entre as provas de ELISA e imunofluorescência indireta (IFI) para detectar anticorpos contra Babesia bovis A comparative study between ELISA and indirect imunofluorescent (IFI) tests to detect antibodies against Babesia bovis
| العنوان: | Estudo comparativo entre as provas de ELISA e imunofluorescência indireta (IFI) para detectar anticorpos contra Babesia bovis A comparative study between ELISA and indirect imunofluorescent (IFI) tests to detect antibodies against Babesia bovis |
|---|---|
| المؤلفون: | João Ricardo Martins, Bartolomeu Lima Corrêa, Victor Hermes Ceresér |
| المصدر: | Ciência Rural, Vol 26, Iss 1, Pp 115-118 (1996) |
| بيانات النشر: | Universidade Federal de Santa Maria, 1996. |
| سنة النشر: | 1996 |
| المجموعة: | LCC:Agriculture LCC:Agriculture (General) |
| مصطلحات موضوعية: | sorologia, Babesia bovis, ELISA, IFI, serology, IFAT, Agriculture, Agriculture (General), S1-972 |
| الوصف: | Comparou-se o desempenho de um kit de ELISA para detectar anticorpos contra Babesia bovis, em hemoparasita bovino, com o teste de Imunofluorescência Indireta (IFI) usualmente empregado em rotina sorológica. Sobre 97 amostras de soros oriundas de uma região livre de carrapatos e hematozoários, o teste de ELISA demonstrou uma especificidade de 98.9% contra 97.9% do teste de IFI. Ambos os testes apresentaram uma sensibilidade de 100% quando utilizados sobre 22 amostras de soro de bovinos experimentalmente infectados com B. bovis. Os resultados obtidos sobre 1560 amostras colhidas à campo, mostraram uma concordância de 90,1% (1406/1560) entre amostras positivas e negativas, enquanto que 4,2% (66) foram positivas para IFI e negativas para ELISA e 5,6% (88) foram negativas para IFI e positivas para ELISA.Performance of an ELISA kit for detecting antibodies to Babesia bovis, a bovine haemoparasite, was compared with the Indirect Fluorescent Antibody Test (IFAT) used in serological routine. Over 97 sera samples from a free tick area, ELISA showed an specificity of 98.9% against 97.9% obtained by IFA T. Both tests showed a sensitivity of 100% when compared over 22 sera samples from experimentally infected bovine with B. bovis. Results obtained with 1560 field samples showed an agreement of 90.1% (1406/1560) between positive and negativo samples while 4.2% (66) were positives to IFAT and negatives to ELISA, and 5.6% (88) owere negatives to IFAT and positives to ELISA. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Portuguese |
| تدمد: | 0103-8478 1678-4596 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84781996000100021; https://doaj.org/toc/0103-8478; https://doaj.org/toc/1678-4596 |
| DOI: | 10.1590/S0103-84781996000100021 |
| URL الوصول: | https://doaj.org/article/4c2b92cf308c451d8c2460ab5f30d08d |
| رقم الانضمام: | edsdoj.4c2b92cf308c451d8c2460ab5f30d08d |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 01038478 16784596 |
|---|---|
| DOI: | 10.1590/S0103-84781996000100021 |