التفاصيل البيبلوغرافية
| العنوان: |
Data integration from pathology slides for quantitative imaging of multiple cell types within the tumor immune cell infiltrate |
| المؤلفون: |
Zhaoxuan Ma, Stephen L. Shiao, Emi J. Yoshida, Steven Swartwood, Fangjin Huang, Michael E. Doche, Alice P. Chung, Beatrice S. Knudsen, Arkadiusz Gertych |
| المصدر: |
Diagnostic Pathology, Vol 12, Iss 1, Pp 1-12 (2017) |
| بيانات النشر: |
BMC, 2017. |
| سنة النشر: |
2017 |
| المجموعة: |
LCC:Pathology |
| مصطلحات موضوعية: |
Quantitative imaging, Tumor immune infiltrate, Immunohistochemistry, Image analysis, Breast cancer, Pathology, RB1-214 |
| الوصف: |
Abstract Background Immune cell infiltrates (ICI) of tumors are scored by pathologists around tumor glands. To obtain a better understanding of the immune infiltrate, individual immune cell types, their activation states and location relative to tumor cells need to be determined. This process requires precise identification of the tumor area and enumeration of immune cell subtypes separately in the stroma and inside tumor nests. Such measurements can be accomplished by a multiplex format using immunohistochemistry (IHC). Method We developed a pipeline that combines immunohistochemistry (IHC) and digital image analysis. One slide was stained with pan-cytokeratin and CD45 and the other slide with CD8, CD4 and CD68. The tumor mask generated through pan-cytokeratin staining was transferred from one slide to the other using affine image co-registration. Bland-Altman plots and Pearson correlation were used to investigate differences between densities and counts of immune cell underneath the transferred versus manually annotated tumor masks. One-way ANOVA was used to compare the mask transfer error for tissues with solid and glandular tumor architecture. Results The overlap between manual and transferred tumor masks ranged from 20%–90% across all cases. The error of transferring the mask was 2- to 4-fold greater in tumor regions with glandular compared to solid growth pattern (p |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1746-1596 |
| Relation: |
http://link.springer.com/article/10.1186/s13000-017-0658-8; https://doaj.org/toc/1746-1596 |
| DOI: |
10.1186/s13000-017-0658-8 |
| URL الوصول: |
https://doaj.org/article/4af4bbb2c5624ba69e04560f997c26aa |
| رقم الانضمام: |
edsdoj.4af4bbb2c5624ba69e04560f997c26aa |
| قاعدة البيانات: |
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