التفاصيل البيبلوغرافية
| العنوان: |
Evaluation of the FPMC respiratory panel for detection of respiratory tract pathogens in nasopharyngeal swab and sputum specimens |
| المؤلفون: |
Li Xue, Jianhong Zhu, Ke Lei, Zeshi Liu, Yiwei Tang, Bo Zhong, Ning Gao, Chaoliang Xiong, Jing Lei, Ying Tian, Weixiao Zhou, Nan Feng, Xue Zhang, Dong Chen, Jing Li, Yan Geng |
| المصدر: |
Virology Journal, Vol 21, Iss 1, Pp 1-7 (2024) |
| بيانات النشر: |
BMC, 2024. |
| سنة النشر: |
2024 |
| المجموعة: |
LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: |
Fluorescent probe melting curve PCR, Respiratory pathogen, Molecular detection, Infectious and parasitic diseases, RC109-216 |
| الوصف: |
Abstract Objectives The performance of the new Respiratory Pathogen panel (fluorescent probe melting curve, FPMC) for the qualitative detection of 12 organisms (chlamydia pneumoniae, mycoplasma pneumoniae, adenovirus, influenza A virus, influenza B virus, parainfluenza virus, rhinovirus, etc.) was assessed. Methods Prospectively collected nasopharyngeal swab (NPS) and sputum specimens (n = 635) were detected by using the FPMC panel, with the Sanger sequencing method as the comparative method. Results The overall percent concordance between the FPMC analysis method and the Sanger sequencing method was 100% and 99.66% for NPS and sputum specimens, respectively. The FPMC testified an overall positive percent concordance of 100% for both NPS and sputum specimens. The FPMC analysis method also testified an overall negative percent concordance of 100% and 99.38% for NPS and sputum specimens, respectively. Conclusions The FPMC analysis method is a stable and accurate assay for rapid, comprehensive detecting for respiratory pathogens. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
1743-422X |
| Relation: |
https://doaj.org/toc/1743-422X |
| DOI: |
10.1186/s12985-024-02430-x |
| URL الوصول: |
https://doaj.org/article/48aed27528254e4da13e76b815a42521 |
| رقم الانضمام: |
edsdoj.48aed27528254e4da13e76b815a42521 |
| قاعدة البيانات: |
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