Academic Journal
Identification and characterization of inorganic-phosphate-solubilizing bacteria from agricultural fields with a rapid isolation method
| العنوان: | Identification and characterization of inorganic-phosphate-solubilizing bacteria from agricultural fields with a rapid isolation method |
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| المؤلفون: | Bang-Xiao Zheng, Muhammad Ibrahim, Ding-Peng Zhang, Qing-Fang Bi, Hong-Zhe Li, Guo-Wei Zhou, Kai Ding, Josep Peñuelas, Yong-Guan Zhu, Xiao-Ru Yang |
| المصدر: | AMB Express, Vol 8, Iss 1, Pp 1-12 (2018) |
| بيانات النشر: | SpringerOpen, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Biotechnology LCC:Microbiology |
| مصطلحات موضوعية: | Phosphorus, Inorganic phosphate solubilizing bacteria, Isolation, Characterization, Biotechnology, TP248.13-248.65, Microbiology, QR1-502 |
| الوصف: | Abstract The ability to solubilize fixed inorganic phosphorus (P) for plant growth is important for increasing crop yield. More P can be released by inoculating soil with inorganic-phosphate-solubilizing bacteria (iPSBs). We used 96-well microplates instead of traditional 200-mm petri dishes to rapidly screen iPSB strains for their solubilizing ability. We simultaneously obtained 76 iPSB isolates from 576 wells containing two agricultural soils. This method conveniently identified positive iPSB strains and effectively prevented fungal cross-contamination. Maximum-likelihood phylogenetic trees of the isolated strains showed that Bacillus megaterium was the most dominant iPSB, and strains Y99, Y95, Y924 and Y1412 were selected as representatives for the analysis of P solubilization. Succinic acid was the main organic acid of B. megaterium for releasing P. It was strongly correlated with the increase in soluble P concentration during 168 h of incubation of these four strains. pH was negatively exponentially correlated with the amount of soluble P in the medium, and the amount of succinic acid was strongly linearly correlated with the amount of P released (P |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2191-0855 |
| Relation: | http://link.springer.com/article/10.1186/s13568-018-0575-6; https://doaj.org/toc/2191-0855 |
| DOI: | 10.1186/s13568-018-0575-6 |
| URL الوصول: | https://doaj.org/article/46eebfea30ae4c3093827cb182000070 |
| رقم الانضمام: | edsdoj.46eebfea30ae4c3093827cb182000070 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 21910855 |
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| DOI: | 10.1186/s13568-018-0575-6 |