Academic Journal
The role of phosphorylation in the elasticity of the tethers that connect telomeres of separating anaphase chromosomes
| العنوان: | The role of phosphorylation in the elasticity of the tethers that connect telomeres of separating anaphase chromosomes |
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| المؤلفون: | Emma Kite, Arthur Forer |
| المصدر: | Nucleus, Vol 11, Iss 1, Pp 19-31 (2020) |
| بيانات النشر: | Taylor & Francis Group, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Genetics LCC:Cytology |
| مصطلحات موضوعية: | anaphase tethers, protein phosphatase 1, protein phosphatase 2a, calyculin a, okadaic acid, crane-fly spermatocytes, anaphase forces, titin, Genetics, QH426-470, Cytology, QH573-671 |
| الوصف: | Elastic tethers, connecting telomeres of all separating anaphase chromosome pairs, lose elasticity when they lengthen during anaphase. Treatment with phosphatase inhibitor CalyculinA causes anaphase chromosomes to move backwards after they reach the poles, suggesting that dephosphorylation causes loss of tether elasticity. We added 50nM CalyculinA to living anaphase crane-fly spermatocytes with different length tethers. When tethers were short, almost all partner chromosomes moved backwards after nearing the poles. When tethers were longer, fewer chromosomes moved backwards. With yet longer tethers none moved backward. This is consistent with tether elasticity being lost by dephosphorylation. 50nM CalyculinA blocks both PP1 and PP2A. To distinguish between PP1 and PP2A we treated cells with short tethers with 50nM okadaic acid which blocks solely PP2A, or with 1µM okadaic acid which blocks both PP1 and PP2A. Only 1µM okadaic acid caused chromosomes to move backward. Thus, tether elasticity is lost because of dephosphorylation by PP1. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1949-1034 1949-1042 19491034 |
| Relation: | https://doaj.org/toc/1949-1034; https://doaj.org/toc/1949-1042 |
| DOI: | 10.1080/19491034.2019.1710329 |
| URL الوصول: | https://doaj.org/article/c46ba565a30a4bc0afaff4abcd9cbc71 |
| رقم الانضمام: | edsdoj.46ba565a30a4bc0afaff4abcd9cbc71 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19491034 19491042 |
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| DOI: | 10.1080/19491034.2019.1710329 |