Academic Journal
Generation of male germ cells from mouse induced pluripotent stem cells in vitro
| العنوان: | Generation of male germ cells from mouse induced pluripotent stem cells in vitro |
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| المؤلفون: | Yangfang Li, Xiuxia Wang, Xue Feng, Shangying Liao, Daoqin Zhang, Xiuhong Cui, Fei Gao, Chunsheng Han |
| المصدر: | Stem Cell Research, Vol 12, Iss 2, Pp 517-530 (2014) |
| بيانات النشر: | Elsevier, 2014. |
| سنة النشر: | 2014 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | Biology (General), QH301-705.5 |
| الوصف: | Germ cells are the only cell type that passes genetic information to the next generation. In most metazoan species, primordial germ cells (PGCs) were induced from epiblasts by signals from the neighboring tissues. In vitro derivation of germ cells from the pluripotent stem cells (PSCs) such as embryonic stem cells (ESCs) and induced PSCs (iPSCs) are of great values for the treatment of infertility, for animal breeding, and for studying the mechanism of germ cell development. Although the derivations of male germ cells from PSCs have been previously reported, most of the studies failed to conduct the induction in a well-controlled and highly efficient manner. Here, we report the derivation of induced PGC-like cells (iPGCLCs) from mouse iPSCs via induced epiblast-like cells (iEpiLCs) as being monitored by the expression of enhanced green fluorescent protein gene under the control of the promoter of stimulated by retinoic acid 8 (Stra8-EGFP). The identity of iPGCLCs was characterized by examining the expression of multiple marker genes as well as by the recovery of spermatogenesis after they were transplanted to the testis of infertile W/Wv mice. Furthermore, iPGCLCs were either induced to germline stem cell-like cells (iGSCLCs) or reverted back to embryonic germ cell-like cells (iEGCLCs). In conclusion, we have established an efficient procedure for inducing iPSCs into iPGCLCs that can be further expanded and induced to more developed germ cells. This work indicates that the technology of in vitro germ cell induction is becoming more sophisticated and can be further improved. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1873-5061 1876-7753 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S1873506113001852; https://doaj.org/toc/1873-5061; https://doaj.org/toc/1876-7753 |
| DOI: | 10.1016/j.scr.2013.12.007 |
| URL الوصول: | https://doaj.org/article/4684ec2d617a462489ff001ad9c67471 |
| رقم الانضمام: | edsdoj.4684ec2d617a462489ff001ad9c67471 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 18735061 18767753 |
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| DOI: | 10.1016/j.scr.2013.12.007 |