التفاصيل البيبلوغرافية
| العنوان: |
Kidney lipids in galactosylceramide synthase-deficient mice: absence of galactosylsulfatide and compensatory increase in more polar sulfoglycolipids1 |
| المؤلفون: |
Keiko Tadano-Aritomi, Toshiyuki Hikita, Hirokazu Fujimoto, Kunihiko Suzuki, Kohji Motegi, Ineo Ishizuka |
| المصدر: |
Journal of Lipid Research, Vol 41, Iss 8, Pp 1237-1243 (2000) |
| بيانات النشر: |
Elsevier, 2000. |
| سنة النشر: |
2000 |
| المجموعة: |
LCC:Biochemistry |
| مصطلحات موضوعية: |
UDP-galactose:ceramide galactosyltransferase, gene targeting, glycolipids, galactosylceramide, sulfatides, sulfolipids, Biochemistry, QD415-436 |
| الوصف: |
UDP-galactose:ceramide galactosyltransferase (CGT) catalyzes the final step in the synthesis of galactosylceramide (GalCer). It has previously been shown that CGT-deficient mice do not synthesize GalCer and its sulfated derivative GalCer I3-sulfate (galactosylsulfatide, SM4s) but form myelin containing glucosylceramide (GlcCer) and sphingomyelin with 2-hydroxy fatty acids. Because relatively high concentrations of GalCer and SM4s are present also in mammalian kidney, we analyzed the composition of lipids in the kidney of Cgt−/− and, as a control, Cgt+/− and wild-type mice. The homozygous mutant mice lacked GalCer, galabiaosylceramide (Ga2Cer), and SM4s. Yet, they did not show any major morphological or functional defects in the kidney. A slight increase in GlcCer containing 4-hydroxysphinganine was evident among neutral glycolipids. Intriguingly, more polar sulfoglycolipids, that is, lactosylceramide II3-sulfate (SM3) and gangliotetraosylceramide II3,IV3-bis-sulfate (SB1a), were expressed at 2 to 3 times the normal levels in Cgt−/− mice, indicating upregulation of biosynthesis of SB1a from GlcCer via SM3. Given that SM4s is a major polar glycolipid constituting renal tubular membrane, the increase in SM3 and SB1a in the mice deficient in CGT and thus SM4s appears to be a compensatory process, which could partly restore kidney function in the knockout mice. —Tadano-Aritomi, K., T. Hikita, H. Fujimoto, K. Suzuki, K. Motegi, and I. Ishizuka. Kidney lipids in galactosylceramide synthase-deficient mice: absence of galactosylsulfatide and compensatory increase in more polar sulfoglycolipids. J. Lipid Res. 2000. 41: 1237–1243. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
0022-2275 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S0022227520334313; https://doaj.org/toc/0022-2275 |
| DOI: |
10.1016/S0022-2275(20)33431-3 |
| URL الوصول: |
https://doaj.org/article/e4549a5298ac4702971ab00d5caf49b9 |
| رقم الانضمام: |
edsdoj.4549a5298ac4702971ab00d5caf49b9 |
| قاعدة البيانات: |
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