Academic Journal
Detection of superoxide dismutase (Cu–Zn) isoenzymes in leaves and pseudobulbs of Bulbophyllum morphologlorum Kraenzl orchid by comparative proteomic analysis
| العنوان: | Detection of superoxide dismutase (Cu–Zn) isoenzymes in leaves and pseudobulbs of Bulbophyllum morphologlorum Kraenzl orchid by comparative proteomic analysis |
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| المؤلفون: | Pattana S. Huehne, Kisana Bhinija, Chantragan Srisomsap, Daranee Chokchaichamnankit, Churat Weeraphan, Jisnuson Svasti, Skorn Mongkolsuk |
| المصدر: | Biochemistry and Biophysics Reports, Vol 22, Iss , Pp - (2020) |
| بيانات النشر: | Elsevier, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Biology (General) LCC:Biochemistry |
| مصطلحات موضوعية: | Bulbophyllum, Orchid, Proteomics, Stress response, Enzymatic antioxidant, Superoxide dismutase (Cu–Zn), Biology (General), QH301-705.5, Biochemistry, QD415-436 |
| الوصف: | Typically, biological systems are protected from the toxic effect of free radicals by antioxidant defense. Extracts from orchids have been reported to show high levels of exogenous antioxidant activity including Bulbophyllum orchids but so far, there have been no reports on antioxidant enzymes. Therefore, differences in protein expression from leaves and pseudobulbs of Bulbophyllum morphologlorum Kraenzl and Dendrobium Sonia Earsakul were studied using two-dimensional gel electrophoresis and mass spectrometry (LC/MS/MS). Interestingly, the largest group of these stress response proteins were associated with antioxidant defense and temperature stress, including superoxide dismutase (Cu–Zn) and heat shock protein 70. The high expression of this antioxidant enzyme from Bulbophyllum morphologlorum Kraenzl was confirmed by activity staining on native-PAGE, and the two Cu/Zn-SODs isoenzymes were identified as Cu/Zn-SOD 1 and Cu/Zn-SOD 2 by LC/MS/MS. The results suggested that Bulbophyllum orchid can be a potential plant source for medicines and natural antioxidant supplements. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2405-5808 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405580820300716; https://doaj.org/toc/2405-5808 |
| DOI: | 10.1016/j.bbrep.2020.100762 |
| URL الوصول: | https://doaj.org/article/40c8e32e78ae4092a58f4637cb796f43 |
| رقم الانضمام: | edsdoj.40c8e32e78ae4092a58f4637cb796f43 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24055808 |
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| DOI: | 10.1016/j.bbrep.2020.100762 |