التفاصيل البيبلوغرافية
| العنوان: |
Nuclear environmental DNA resolves fine-scale population genetic structure in an aquatic habitat |
| المؤلفون: |
Zifang Liu, Mary A. Kishe, Nestory P. Gabagambi, Asilatu H. Shechonge, Benjamin P. Ngatunga, Katie Smith, Andrew D. Saxon, Alan G. Hudson, Tyler Linderoth, George F. Turner, Rupert A. Collins, Martin J. Genner |
| المصدر: |
iScience, Vol 27, Iss 1, Pp 108669- (2024) |
| بيانات النشر: |
Elsevier, 2024. |
| سنة النشر: |
2024 |
| المجموعة: |
LCC:Science |
| مصطلحات موضوعية: |
Environmental science, Genetics, Techniques in genetics, Evolutionary biology, Science |
| الوصف: |
Summary: There is considerable potential for nuclear genomic material in environmental DNA (eDNA) to inform us of population genetic structure within aquatic species. We tested if nuclear allelic composition data sourced from eDNA can resolve fine scale spatial genetic structure of the cichlid fish Astatotilapia calliptera in Lake Masoko, Tanzania. In this ∼35 m deep crater lake the species is diverging into two genetically distinguishable ecomorphs, separated by a thermo-oxycline at ∼15 m that divides biologically distinct water masses. We quantified population genetic structure along a depth transect using single nucleotide polymorphisms (SNPs) derived from genome sequencing of 530 individuals. This population genetic structure was reflected in a focal set of SNPs that were also reliably amplified from eDNA — with allele frequencies derived from eDNA reflecting those of fish within each depth zone. Thus, by targeting known genetic variation between populations within aquatic eDNA, we measured genetic structure within the focal species. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
2589-0042 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S2589004223027463; https://doaj.org/toc/2589-0042 |
| DOI: |
10.1016/j.isci.2023.108669 |
| URL الوصول: |
https://doaj.org/article/4062208fc4a84bf2ade03d01471fb1a9 |
| رقم الانضمام: |
edsdoj.4062208fc4a84bf2ade03d01471fb1a9 |
| قاعدة البيانات: |
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