Academic Journal
Potential anti-viral compounds from Malaysian Plant Natural Product Repository and Database (MyNature50000) for DENV2
| العنوان: | Potential anti-viral compounds from Malaysian Plant Natural Product Repository and Database (MyNature50000) for DENV2 |
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| المؤلفون: | Iffah Izzati Zakaria, Nurul Hanim Salin, Azimah Amanah, Shatrah Othman, Farahayu Khairuddin, Muhammad Hidhir Khawory, Roswanira Abdul Wahab, Mohd Ridzuwan Abdul Rahaman, Pua Pei Chern, Nor Azfa Johari, Habibah Wahab |
| المصدر: | Biotechnology & Biotechnological Equipment, Vol 33, Iss 1, Pp 379-389 (2019) |
| بيانات النشر: | Taylor & Francis Group, 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Biotechnology |
| مصطلحات موضوعية: | natural products, high-throughput, antiviral agents, dengue drug discovery, flavivirus drug discovery, Biotechnology, TP248.13-248.65 |
| الوصف: | Dengue virus (DENV) is a globally significant human pathogen that annually infects more than ∼300 million of the Earth’s population. While the clinically approved anti-dengue vaccine, Dengvaxia is available for treating such infection, its treatment is limited to those infected with DENV of serotypes 3 and 4 only. However, the vaccine is no longer available to treat dengue infections following the suspended use of Dengvaxia after several discoveries on the side effects of the vaccine on humans. In view of this recent development, a collection of plant extracts in the MyNature50000 library showing potential anti-dengue activities were explored. In this work, the DENV2 NS2B-NS3 bioassay was setup and optimized to evaluate the potential inhibitory activity of plant extracts stored in the MyNature50000 natural product collections. A total of 150 plants were examined using the high-throughput screening assay, among which four candidate plants showed potential as inhibitors of the DENV2 NS2B-NS3 protease, with exceptional inhibition activity >95% with corresponding IC50 values ranging within 2 − 5 µg/mL. Two local plants, Syzygium campanulatum (‘Kelat Paya’) and Syzygium grande (‘Keriang Batu’), were phytochemically analyzed and the data revealed existence of cyclododecane, n-hexadecanoic acid and caryophyllene, all of which are compounds well-known for their inhibitory activity against DENV2 NS2B-NS3. Additionally, MTT assays showed that both plants were mildly cytotoxic on mammalian cell lines, with IC50 values >20 µg/mL, indicating their prospective use as drugs to combat DENV. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1310-2818 1314-3530 13102818 |
| Relation: | https://doaj.org/toc/1310-2818; https://doaj.org/toc/1314-3530 |
| DOI: | 10.1080/13102818.2019.1578184 |
| URL الوصول: | https://doaj.org/article/400b1665825c4f219e12562f2461e84e |
| رقم الانضمام: | edsdoj.400b1665825c4f219e12562f2461e84e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 13102818 13143530 |
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| DOI: | 10.1080/13102818.2019.1578184 |