Academic Journal
Target-responsive aptamer-cross-linked hydrogel sensors for the visual quantitative detection of aflatoxin B1 using exonuclease I-Triggered target cyclic amplification
| العنوان: | Target-responsive aptamer-cross-linked hydrogel sensors for the visual quantitative detection of aflatoxin B1 using exonuclease I-Triggered target cyclic amplification |
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| المؤلفون: | Mengyao Zheng, Hongmei Liu, Jin Ye, Baoxia Ni, Yanli Xie, Songxue Wang |
| المصدر: | Food Chemistry: X, Vol 15, Iss , Pp 100395- (2022) |
| بيانات النشر: | Elsevier, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Nutrition. Foods and food supply LCC:Food processing and manufacture |
| مصطلحات موضوعية: | Hydrogel, Enzyme cascade, Aptamer, AFB1, Visual detection, Nutrition. Foods and food supply, TX341-641, Food processing and manufacture, TP368-456 |
| الوصف: | For the on-site detection of aflatoxin B1 (AFB1), a DNA hydrogel was prepared as a biosensor substrate, while an AFB1 aptamer was used as the recognition element. An AFB1-responsive aptamer-cross-linked hydrogel sensor was constructed using an enzyme-linked signal amplification strategy; AFB1 binds competitively to the aptamer, causing the hydrogel to undergo cleavage and release horseradish peroxidase (HRP). The addition of exonuclease I (ExoI) to the hydrogel causes the release of AFB1 from the aptamer, promoting additional hydrogel cleavage to release more HRP, ultimately catalysing the reaction between 3,3′,5,5′-tetramethylbenzidine and H2O2. The hydrogel sensor exhibited an outstanding sensitivity (limit of detection, 4.93 nM; dynamic range, 0–500 nM), and its selectivity towards seven other mycotoxins was confirmed. The feasibility and reliability were verified by measuring the AFB1 levels in peanut oil (recoveries, 89.59–95.66 %; relative standard deviation, |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2590-1575 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2590157522001936; https://doaj.org/toc/2590-1575 |
| DOI: | 10.1016/j.fochx.2022.100395 |
| URL الوصول: | https://doaj.org/article/3f120f2fecb04263be133d39263176ad |
| رقم الانضمام: | edsdoj.3f120f2fecb04263be133d39263176ad |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 25901575 |
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| DOI: | 10.1016/j.fochx.2022.100395 |