التفاصيل البيبلوغرافية
| العنوان: |
Evaluating the Anticancer Potential of Ornithogalum saundersiae Saponins on Hepatocellular Carcinoma: A Transcriptome Sequencing Method |
| المؤلفون: |
Bo XU, Ting JIANG, Wangde JIN, Xinglin JIN |
| المصدر: |
Shipin gongye ke-ji, Vol 45, Iss 24, Pp 346-352 (2024) |
| بيانات النشر: |
The editorial department of Science and Technology of Food Industry, 2024. |
| سنة النشر: |
2024 |
| المجموعة: |
LCC:Food processing and manufacture |
| مصطلحات موضوعية: |
tiger's eye saponin, transcriptome sequencing, hep3b cells, differentially expressed genes, Food processing and manufacture, TP368-456 |
| الوصف: |
To investigate the crucial pathways and differentially expressed genes following the action of Ornithogalum saundersiae Saponins (OSW-1) on liver cancer cells (HEP3B), this study utilized transcriptome sequencing technology to examine the gene expression data of HEP3B cells treated with OSW-1. By precisely analyzing the transcripts from the measured sequence information, differentially expressed genes related to cell survival were screened to identify core genes. Furthermore, experiments were conducted to verify whether OSW-1 could treat hepatocellular carcinoma by regulating the core targets. Results showed that the MTT assay revealed a significant reduction in HEP3B cell activity following OSW-1 treatment, which was dependent on the concentration. A total of 1381 genes showed significant differences in expression as a result of transcriptome sequencing, with a majority being involved in the TNF signaling pathway. Additionally, immunofluorescence and Western blot analysis indicated that the levels of p-PI3K, p-AKT, and p-NF-κB proteins were reduced in HEP3B cells after treatment with OSW-1. In conclusion, OSW-1 may suppress inflammation and reduce HEP3B cell activity by modulating inflammatory signaling pathways, suggesting its potential as a drug for liver cancer treatment and providing an experimental foundation for the development of healthy foods aimed at liver protection. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
Chinese |
| تدمد: |
1002-0306 |
| Relation: |
https://doaj.org/toc/1002-0306 |
| DOI: |
10.13386/j.issn1002-0306.2023120158 |
| URL الوصول: |
https://doaj.org/article/3e657f213ce1484588c24292889e51d7 |
| رقم الانضمام: |
edsdoj.3e657f213ce1484588c24292889e51d7 |
| قاعدة البيانات: |
Directory of Open Access Journals |