Academic Journal
Molecular evidence of high-risk human papillomavirus infection in colorectal tumours from Cuban patients
| العنوان: | Molecular evidence of high-risk human papillomavirus infection in colorectal tumours from Cuban patients |
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| المؤلفون: | Yudira Soto, Celia Maria Limia, Licet González, Bienvenido Grá, Olga Marina Hano, Pedro Ariel Martínez, Vivian Kourí |
| المصدر: | Memorias do Instituto Oswaldo Cruz, Iss 0 |
| بيانات النشر: | Fundação Oswaldo Cruz (FIOCRUZ). |
| المجموعة: | LCC:Microbiology LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | human papillomavirus, colorectal cancer, real-time PCR, Microbiology, QR1-502, Infectious and parasitic diseases, RC109-216 |
| الوصف: | The association between colorectal cancer and human papillomavirus (HPV) infection is still unproven. The aim of this study was to investigate the presence of high-risk HPV (HR-HPV) DNA in colorectal tissues from Cuban patients. A total of 63 colorectal formalin-fixed paraffin-embedded tissues were studied (24 adenocarcinoma, 18 adenoma, and 21 colorectal tissues classified as benign colitis). DNA from colorectal samples was analysed by quantitative real-time polymerase chain reaction to detect the most clinically relevant high HR-HPV types (HPV-16, -18, -31, -33, -45, -52, and -58). Associations between histologic findings and other risk factors were also analysed. Overall, HPV DNA was detected in 23.8% (15/63) of the samples studied. Viral infections were detected in 41.7% of adenocarcinoma (10/24) and 27.7% of adenoma cases (5/18). HPV DNA was not found in any of the negative cases. An association between histological diagnosis of adenocarcinoma and HPV infection was observed (odd ratio = 4.85, 95% confidence interval = 1.40-16.80, p = 0.009). The only genotypes identified were HPV 16 and 33. Viral loads were higher in adenocarcinoma, and these cases were associated with HPV 16. This study provides molecular evidence of HR-HPV infection in colorectal adenocarcinoma tissues from Cuban patients. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1678-8060 0074-0276 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762016005022102&lng=en&tlng=en; https://doaj.org/toc/1678-8060 |
| DOI: | 10.1590/0074-02760160217 |
| URL الوصول: | https://doaj.org/article/3d3bd2bc69804d5e929c5d4668071e43 |
| رقم الانضمام: | edsdoj.3d3bd2bc69804d5e929c5d4668071e43 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 16788060 00740276 |
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| DOI: | 10.1590/0074-02760160217 |