Academic Journal
Mineralization of acephate, a recalcitrant organophosphate insecticide is initiated by a pseudomonad in environmental samples.
| العنوان: | Mineralization of acephate, a recalcitrant organophosphate insecticide is initiated by a pseudomonad in environmental samples. |
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| المؤلفون: | Aleem Basha Pinjari, Boris Novikov, Yohannes H Rezenom, David H Russell, Melinda E Wales, Dayananda Siddavattam |
| المصدر: | PLoS ONE, Vol 7, Iss 4, p e31963 (2012) |
| بيانات النشر: | Public Library of Science (PLoS), 2012. |
| سنة النشر: | 2012 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | An aerobic bacterium capable of breaking down the pesticide acephate (O,S-dimethyl acetyl phosphoramidothioic acid) was isolated from activated sludge collected from a pesticide manufacturing facility. A phylogenetic tree based on the 16 S rRNA gene sequence determined that the isolate lies within the Pseudomonads. The isolate was able to grow in the presence of acephate at concentrations up to 80 mM, with maximum growth at 40 mM. HPLC and LC-MS/MS analysis of spent medium from growth experiments and a resting cell assay detected the accumulation of methamidophos and acetate, suggesting initial hydrolysis of the amide linkage found between these two moieties. As expected, the rapid decline in acephate was coincident with the accumulation of methamidophos. Methamidophos concentrations were maintained over a period of days, without evidence of further metabolism or cell growth by the cultures. Considering this limitation, strains such as described in this work can promote the first step of acephate mineralization in soil microbial communities. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | http://europepmc.org/articles/PMC3319554?pdf=render; https://doaj.org/toc/1932-6203 |
| DOI: | 10.1371/journal.pone.0031963 |
| URL الوصول: | https://doaj.org/article/3b22cf6feb2746ba8e5b1058250dae74 |
| رقم الانضمام: | edsdoj.3b22cf6feb2746ba8e5b1058250dae74 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0031963 |