التفاصيل البيبلوغرافية
| العنوان: |
LC-MS based simultaneous profiling of adrenal hormones of steroids, catecholamines, and metanephrines |
| المؤلفون: |
Jongsung Noh, Chaelin Lee, Jung Hee Kim, Seung Woon Myung, Man Ho Choi |
| المصدر: |
Journal of Lipid Research, Vol 64, Iss 11, Pp 100453- (2023) |
| بيانات النشر: |
Elsevier, 2023. |
| سنة النشر: |
2023 |
| المجموعة: |
LCC:Biochemistry |
| مصطلحات موضوعية: |
adrenal tumor, chemical derivatization, mass spectrometry, adrenal cortex, adrenal medullar, Biochemistry, QD415-436 |
| الوصف: |
Metabolic changes in adrenocortical steroids and medullary catecholamines characterize adrenal tumors, but they are measured using different analytical protocols. To increase bioanalytical validity while maintaining sample homogeneity, LC-MS-based profiling of 29 cortical steroids and 6 medullary amines, including catecholamines and metanephrines, in a single run was developed. Alkyloxycarbonylation with isobutyl chloroformate was employed together with our comprehensive steroid assay, and all adrenal hormones were separated on a reversed-phase C18 column (50 × 2.1 mm, 1.9 μm) at a flow rate of 0.3 ml/min. The lower limits of quantification for all analytes ranged from 0.1 to 2.0 ng/ml, with extraction recoveries of 58.5%–109.5%, while the imprecision and accuracy were 1.6%–14.8% and 89.2%–114.9%, respectively. The validated LC-MS assay was applied to serum samples obtained from 60 patients with adrenal Cushing syndrome, primary aldosteronism, and pheochromocytoma/paraganglioma (PPGL). In addition to the characteristic metabolic changes in glucocorticoids, mineralocorticoids, catecholamines, and metanephrine, the molecular ratios of dehydroepiandrosterone sulfate and 20α-dihydrocortisol indicated Cushing syndrome and primary aldosteronism (P < 0.01 for all compounds), respectively. Moreover, the interactive molecular ratios of 11-deoxycortisol with normetanephrine, metanephrine, norepinephrine, and epinephrine (P < 0.01 all compounds) were proposed to characterize the metabolic features of PPGL. Novel LC-MS-based quantitative profiling of steroids, catecholamines, and metanephrines in human serum was successfully established and characterized metabolic features of individual adrenal tumors that could be used for clinical purposes. |
| نوع الوثيقة: |
article |
| وصف الملف: |
electronic resource |
| اللغة: |
English |
| تدمد: |
0022-2275 |
| Relation: |
http://www.sciencedirect.com/science/article/pii/S0022227523001268; https://doaj.org/toc/0022-2275 |
| DOI: |
10.1016/j.jlr.2023.100453 |
| URL الوصول: |
https://doaj.org/article/d3b028095c0e492dac5ece6bb37351c4 |
| رقم الانضمام: |
edsdoj.3b028095c0e492dac5ece6bb37351c4 |
| قاعدة البيانات: |
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