Academic Journal
Association of Hypoxia-inducible factor α subunits with TSGA10 transcripts in HeLa, MCF7 and MDA-MB231- cell lines
| العنوان: | Association of Hypoxia-inducible factor α subunits with TSGA10 transcripts in HeLa, MCF7 and MDA-MB231- cell lines |
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| المؤلفون: | Parya Rahmani Rad, Maryam Beigom Mobasheri, Morteza Hashemzadeh-Chaleshtori, Mohammad Hossein Modarressi |
| المصدر: | Basic & Clinical Cancer Research, Vol 9, Iss 3 (2018) |
| بيانات النشر: | Tehran University of Medical Sciences, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | TSGA10, HIF-α subunits, Cancer-Testis genes, breast cancer cell lines, MCF7, MDA-MB-231, Medicine |
| الوصف: | Background: Hypoxia is a common phenomenon in cancer cells, related to angiogenesis and cell proliferation the hypoxia-inducible factor family (HIFs) is the primary transcriptional factor to hypoxic stress. Cancer-testis (CT) antigens are almost expressed in male germ cells, aberrantly expresses in some malignancies as well. The CT gene, TSGA10, prevents the nuclear accumulation of HIF-α and may be involved in organ-specific regulation of hypoxic gene expression during sperm maturation. TSGA10 is supposed to regulate the HIF expression in germ cells and cancer cells. The HIF-α subunit has three isoforms, involved in oxygen transport, angiogenesis and tumor metastasis, which their detection is the subject of the current study. Methods: Three cell lines, MCF7, MDA-MB-231 and HeLa were cultured, passaged and categorized into normal and synchronized groups. The cells were subjected to RNA extraction and reverse-transcribed into cDNAs. Real time RT-PCR was performed to amplify TSGA10 and HIF-α isoforms and HPRT, as the normalizer gene, using appropriate primers. The REST and SPSS software were used for statistical analysis. Results: The expression of three isoforms of HIF-α in HeLa cell line was higher than MCF7, and MCF7 was higher than MDA-MB-231. Moreover, the expression relationship between HIF-α isoforms and TSGA10 was evaluated in each three cell lines as well. The results were significant in all cases with P =0.01. Before and after synchronization in each three cell lines, the isoform expressions of HIF-α and TASGA10 were evaluated, and the results were revealed their dependent expression. The relationship between HIF-α isoforms and TSGA10 expression was compared with each other. The cell lines with less TSGA10 expression had the higher expression of HIF-α isoforms and vice versa, according to the extent of TSGA10. Conclusion: The significant relationship between expressions of TSGA10 and HIF-α isoforms is confirmed. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2228-6527 2228-5466 |
| Relation: | https://bccr.tums.ac.ir/index.php/bccrj/article/view/264; https://doaj.org/toc/2228-6527; https://doaj.org/toc/2228-5466 |
| URL الوصول: | https://doaj.org/article/e3affa15f7b2484393cfdef39d78983f |
| رقم الانضمام: | edsdoj.3affa15f7b2484393cfdef39d78983f |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 22286527 22285466 |
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