Academic Journal
MOLECULAR DETECTION, ISOLATION, AND PATHOLOGY OF BOVINE TUBERCULOSIS IN AN ORGANIZED FARM IN ASSAM, INDIA
| العنوان: | MOLECULAR DETECTION, ISOLATION, AND PATHOLOGY OF BOVINE TUBERCULOSIS IN AN ORGANIZED FARM IN ASSAM, INDIA |
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| المؤلفون: | Nur Abdul Kader, Samir Das, Acheenta Gohain Barua, Biswajit Dutta, Razibuddin Ahmed Hazarika, Nagendra Nath Barman, Sayed Nabil Abedin, Syed Abdul Arif, Pranjal Moni Nath, Uttam Rajkhowa |
| المصدر: | Exploratory Animal and Medical Research, Vol 12, Iss 1, Pp 46-53 (2022) |
| بيانات النشر: | West Bengal Veterinary Alumni Association, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Veterinary medicine |
| مصطلحات موضوعية: | acid fast, bovine, histopathology, is6110-pcr, sictt, tuberculosis, Veterinary medicine, SF600-1100 |
| الوصف: | Bovine tuberculosis (bTB) is a well-known zoonotic disease that affects cattle all over the world and results in significant economic loss, particularly in impoverished nations. The present communication describes the pathology, isolation, and molecular detection of Mycobacterium in an organized farm in Assam which has previous records on animals with confirmed M. bovis infection. During the period 2020-2021, a total of 40 animals (4 males and 36 females) of one year and above were included in the present study for screening of bovine tuberculosis by single intradermal comparative tuberculin test (SICCT). The milk, nasal swabs were collected from only tuberculin positive cattle and the tissue samples from necropsied animal and then processed for bacteriology, histopathology, and molecular detection from direct samples. Out of 40, four cows showed positive reactors by SICCT, and out of these four, one animal died. At necropsy, there was the presence of circumscribed yellowish-white lesions of various sizes and numbers. The smear prepared from granulomatous tissue samples showed the presence of acid-fast bacilli by Ziehl–Neelsen stain. Mycobacterium could be isolated from tissue samples. The DNA extracted from the samples could amplify Mycobacteria genus specific hsp65 gene and MTBC specific a 123-bp segment of the insertion sequence IS6110. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2277-470X 2319-247X |
| Relation: | https://www.animalmedicalresearch.org/Vol.12_Issue-1_June_2022/MOLECULAR%20DETECTION.pdf; https://doaj.org/toc/2277-470X; https://doaj.org/toc/2319-247X |
| DOI: | 10.52635/eamr/12.1.46-53 |
| URL الوصول: | https://doaj.org/article/a3ae6a79257443148995a43f8d31b8f1 |
| رقم الانضمام: | edsdoj.3ae6a79257443148995a43f8d31b8f1 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 2277470X 2319247X |
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| DOI: | 10.52635/eamr/12.1.46-53 |